Antimicrobia Effect of Aloe vera and Chitosan " in vitro study "

Since the infectious and toxic diseases constitute a wide range of diseases as well as the number of antibiotic-resistant microbial strains is increasing, a natural, new and safe antimicrobials is greatly required. The aim of this study is to investigate the antimicrobial effect of Aloe vera and Chitosan on Streptococcus pyogenes PTCC 1447, Klebsiella pneumoniae PTCC 1053, Staphylococcus aureus PTCC 1337 and Escherichia coli PTCC 1330 and its comparison with common therapeutic antibiotics. To evaluate the antimicrobial activity, Disc diffusion test with Kirby-Bauer method was used. The Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) were determined by using the dilution method. Statistical analysis was carried out by analysis of variance (ANOVA). The highest zone of growth inhibition in 40 mg/ml concentration was related to Streptococcus pyogenes and the minimum zone diameter in this concentration was related to the Gram-negative bacteria, Klebsiella pneumoniae. MIC of Aloe vera for Streptococcus pyogenes, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli was 2, 8, 4 and 8 mg/ ml and their MBC of the was 4, 16, 8 and 8 mg/ml, respectively. MIC of Chitosan for Streptococcus pyogenes, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli was 2, 4, 4 and 8 mg/ ml and their MBC of the extract was 2, 8, 8 and 8 mg/ml, respectively. Aloe vera and Chitosan in comparison with common therapeutic antibiotics had more inhibitory effect on some of the studied strains in vitro.