Identification of Virulence Gene Panton-Valentine Leukocidin (PVL) and Resistance to Methicillin (mecA) in Staphylococcus Aureus Isolated from Clinical Specimens: A Short Report

Staphylococcus aureus is a major pathogen of human in the world. Methicillin-resistant Staphylococcus aureus is associated with nosocomial infections. Recently, staphylococcal infections have been associated with the community. The purpose of this study was to isolate and identify Staphylococcus aureus virulence Panton-Valentine leukocidin (PVL) and methicillin resistance genes in clinical samples using PCR techniques.In this experimental study after collecting samples, it was performed biochemical and microbial tests and then antibiotic susceptibility testing by disk diffusion method according to CLSI guidelines with antibiotics of different groups. To identify the virulence and resistance genes multiplex PCR test was used. The findings showed that the prevalence of methicillin-resistant gene (mecA) in clinical samples are 50% and PVL 2.5%, respectively. Among the antibiotics, resistance to azithromycin, linezolid, ofloxacin and methicillin were 32.5%, 35%, 27.5% and 27.5%, respectively. There is a serious threat for public health because of the importance of Staphylococcus aureus as a major pathogen in developing countries and due to the increasing use of antibacterial agents. Multiplex PCR is a simple, sensitive, inexpensive, relatively quick, and very unique test to identify multiple of genes, simultaneously.