فهرست مطالب k. amini

Birth injuries can be defined as a disruption in the function of the baby's body due to adverse events during birth, which can be avoidable or unavoidable. Despite careful antenatal care, birth trauma usually occurs in long and difficult labors or fetal malformations, although it may also occur in deliveries that are initially unproblematic. Therefore, the purpose of the present study was to determine and investigate the causes of newborn injuries in childbirth processes in the cases referred to the legal medical department of Kohgiluyeh and Boyer Ahmad province, Iran, during the years 2010-2020.

The present retrospective cross-sectional descriptive study was conducted with the aim of investigating newborn injuries during childbirth. Its statistical population includes all complaint cases in the fields of gynecology and midwifery in the Forensic Medicine Department of Kohgiluyeh and Boyar Ahmad provinces during 10 years (2010-2020). The data collection tool included a checklist and a form containing the desired information, which was prepared and completed by the researcher using the files referred to the Department of Legal Medical Commissions. The collected data were analyzed using chi-square statistical tests.

The results indicated that 58 cases were investigated, 39 babies died at birth or shortly after birth and the reason for their complaint was the death of the baby, 19 babies were born alive and the reason for their complaint was birth injury. Infant injuries include; hypoxic-ischemic as the most common injury; Neuro-skeletal injuries, which include Orb-Duchen's paralysis as the most common injury in this group, and bone fractures, including; Fractures were humerus, clavicle, scapula and femur. 39 babies died, of which 10 babies had nerve damage, 8 people died of meconium aspiration, 4 people died due to asphyxia and other cases, moreover the results exposed that one person had a bruised tissue damage.

The results of the present study indicated that the causes of infant mortality and the disorders that occur in them can be influenced by various factors, and by identifying the cause of errors and omissions, preventable maternal and infant complications can be reduced.

In the present study, friction stir process (FSP) was used to produce AL/ZrO2/ZrSiO4 surface hybrid composite at a fixed rotation speed of 1400 rpm and traverse speeds of 20, 25, 31.5 and 40 mm/min. Therefore, the purpose of the mentioned study is to investigate the effect of tool traverse speed on the microstructure, hardness and wear behavior of the above-mentioned surface hybrid composite and compare it with base material aluminum 5052. Investigations showed that as a result of FSP operation, a fine-grained structure is created, which improves the hardness and wear resistance of the samples compared to the base sample with the presence of ZrO2 and ZrSiO4 particles. Also, the results showed that among the FSP samples, the sample with a speed of 20 mm/min has the highest hardness and wear resistance. The reason for this is that in this sample, due to the lower traverse speed compared to other samples, more heat has been generated, which has led to more suitable particle distribution and more fine particles. Therefore, in the sample with the traverse speed of 20 mm/min, the hardness and wear resistance increases by 27.3% and 68.9% respectively compared to the base material sample. Also, the examination of the wear surfaces of the samples showed that the wear mechanism in the base sample is strong adhesive wear, and as a result of the FSP operation and surface compositing due to the fineness of the grains and the increase in hardness, the wear mechanism has become weak adhesive, so the wear resistance of the sample is FSPs have been improved.

The present reserach aimed to detect and isolate the genes involved in the staphylococcal enterotoxins (SEs) production in strains isolated from unprocessed cow’s milk and to examine the impact of Bifidobacterium bifidum probiotic cell-free supernatant (CFS) on their expression. Standard techniques were used for isolation and identification of Staphylococci strains in unprocessed milk. The PCR was used to identify strains carrying enterotoxin genes. The B. bifidum CFS was applied to strains containing the target genes, and the genes expression levels were quantified using Real-time PCR. Using 16SrDNA sequencing, the phylogenic relationship of the isolated strains was determined. Analysis revealed that bacteria such as Staphylococcus species were found in the 72% of the samples. The PCR test showed the presence of various SE superantigens, including SEA (16.7%), SEC (11.7%), SED (8.3%), SEE (6.7%), and SEB (1.7%) in isolated strains. The B. bifidum CFS had obvious antimicrobial activity against strains 24, 51, 54, and 35 of Staphylococcus species, and the minimum inhibitory concentration and minimum bactericidal concentration values for these strains treated with B. bifidum CFS were in the range of 31.25-125 μg/ml. Strains 51 and 24 were clustered with S.aureus ATCC 25923, and strains 54 and 35 were clustered with S.aureus ATCC 12600, respectively. The RT-PCR exhibited that probiotics CFS suppressed the expression of SEA, SEB, SEC, and SEE genes (P<0.05). The average fold change for SEA, SEB, SEC, and SED genes was -1.681, -1.28, -1.52, and -0.84, respectively. The research demonstrated that probiotic bacteria can lower enterotoxin production by downregulating the expression of SEs genes.

Isolation and identification of probiotic strains from native foods can lead to finding strains with unique capabilities. The aim of this study was to isolate, identify and evaluate the ability of Lactobacillus acidophilus bacteria isolated from traditional Tarkhineh food in lowering cholesterol and triglyceride levels in vitro. Native strains of Lactobacillus acidophilus were isolated from a local food called Tarkhineh using culture medium and identified based on phenotypic characteristics, standard biochemical tests and 16SrRNA sequencing. In the next step, the strains were examined for probiotic properties such as resistance to acid and bile and sensitivity to antibiotics. Cholesterol and triglyceride lowering activity of isolates in culture medium was also investigated by o-phthaldehyde method. The results showed that 16 strains of Lactobacillus acidophilus were isolated from Tarkhineh samples, among which four strains (S3, S12, S6, S5) showed 30 to 50% resistance, three strains (S7, S4, S10). had 50 to 75% resistance and 2 strains (S11, S1) had 75 to 100% growth in acidic MRS. In addition, strains S7 and S1 showed a growth rate of 0.3 to 0.5% and strains S4, S3, S11 grew at 0.7% bile salt in MRS medium. The highest cholesterol uptake was observed in S11 and S1 strains at 64± 0.23 and 60±0.18 (p<0.01) respectively. Strains S1 and S11 applied 72% ± 0.64 and 61 ± 0.25% triglyceride reduction respectively. The data of this study showed that the native strains of Lactobacillus acidophilus isolated from the local food of Tarkhineh had the potential to absorb and reduce cholesterol and triglycerides of the culture medium. These strains can be studied in laboratory animals as probiotics capable of absorbing and removing fat.

Clostridium novyi (C. novyi) causes deadly Black disease in sheep and rarely in other animals. Alpha toxin (α-toxin), the most apparent pathogen of this disease, is produced by C. novyi type B. Economic damages of C. novyi include sheep mortality costs, depreciation of affected farms, and health problems with infected carcasses. The identification of C. novyi and isolation of its pathogens by conventional methods is a time-consuming process, necessitating a simple and rapid method for isolating and detecting pathogenic C. novyi. Therefore, this study aimed to molecularly identify α-toxin in local C. novyi isolates from the sheep livers. In this study, 75 livers suspected of Black disease were sampled. The samples of the liver were cultured under anaerobic conditions. Some of the cultured colonies were used in biochemical tests. For molecular confirmation, the DNA of isolates was extracted, and the isolates were confirmed by the polymerase chain reaction (PCR) on the liver tissue and cultured samples using specific α-toxin primers. The PCR on α-toxin produced a band in the range of 609 bp, indicating that the samples belonged to C. novyi. According to the results, of 75 isolates, 18 isolates were confirmed as C. novyi. C. novyi type B was isolated from the liver and confirmed by biochemical and molecular characterization. The PCR assay ensured a sensitive and specific tool for the detection of C. novyi in the samples.

 The high quality of nursing care for cancer patients is a shared priority for health policymakers. Two important influencing variables on the quality of nursing care are the ethics climate governing hospitals and missed nursing care. This study aimed to determine the relationship between ethical climates and missed nursing care in centers providing services to cancer patients in Rasht and Zanjan.

This cross-sectional study was conducted in 2019. The participants were 360 patients with cancer and 84 nurses working in the oncology wards of Rasht and Zanjan, who were included in the study using the Poisson distribution sampling method. The data collection tools were two standard scales; the Miss Care survey and Ethical Climate Questionnaire, and two demographic forms specific to patients and nurses. SPSS version 16 statistical software and independent t-tests, and Pearson's correlation were used to analyze the data.

The results showed that the dominant ethical climate in the mentioned care units was professional and law-oriented, and the independent ethical climate was at the bottom. The mean score of missed nursing care is 1.95±0.40 out of 5. According to the Pearson correlation test, there was a significant negative relationship between ethical climate and missed nursing care in cancer care units (r=-0.422, p<0.001).

A negative and significant relationship between the ethical climate and missed nursing care showed that nurses' correct perception of the ethical climate reduces missed nursing care. It seems that the nursing care quality can be improved by preparing and setting up care guidelines, rules and clear care instructions.

Tracheostomy is one of the most common surgeries in patients with upper airway defects. Tracheostomy is a surgical procedure to open a side passage through the neck into the trachea to stabilize the airway. The complications of this operation are very important, common and at the same time preventable. The purpose of this article is to report a case of a fractured fragment of tracheostomy tube entering the left bronchus.

 The patient was a 34-year-old man diagnosed with amyotrophic lateral sclerosis and quadriplegia, who has had a tracheostomy tube for 7 years, who referred with complaint of shortness of breath following a tracheostomy tube fracture. The patient had 60% saturation and significant respiratory distress. After confirming the fracture of the tracheostomy tube with a CT scan of the lung, the fractured fragment was removed with rigid bronchoscopy and the tracheostomy tube was re-inserted for the patient. The patient was discharged from the hospital two days after tracheostomy.

In case of shortness of breath and decreased saturation, tracheostomy tube fracture should be considered. Proper cleaning and planned replacement of the tracheostomy tube may prevent this complication.

The invA gene plays a critical role in the pathogenicity of Salmonella infantis. Marine organisms, including sea cucumbers due to their effective secondary metabolites, have been identified and studied with compounds with antioxidant, anti-cancer, antibacterial and anti-inflammatory properties. The aim of this study is to determine effect of sea cucumber extraction (Holothuria leucospilota)  on S. infantis invA gene expression .

Poultry meat was sampled. The S. infantis strains containing invA gene were isolated. The Hexane extract was extracted from sea cucumber colon tissue. Its effects on S. infantis and its effect on gene expression were investigated by MIC and Real-time PCR, respectively.

Morphological and biochemical characteristics of these bacteria were confirmed. From 450 samples, 12 S.infantis isolates were isolated. The PCR technique was used to identify the invA encoding gene. All 12 isolates have invA virulence genes. MIC was determined 256 µg/ml. The effect of sea cucumber extract on invA gene expression in S.infantis was evaluated, and the rate of change for the invA gene is estimated -1.21.

According to our results, hexane extract extracted from the sea cucumber (H. leucospilota) caused reduction ofinvA gene expression in salmonella infantis. So, it can be used as a therapeutic supplement against S. infantis

The antimicrobial activity of brown alga methanol, ethyl acetate, hexane, and chloroform extracts on gram positive and gram negative bacteria, and fungi was evaluated by using nutrient broth macro dilution test. Sargassum glaucescense was collected around the coastal area of Chabahar (south of Iran) the protected marine area of the Oman Sea in April and May 2015. Six pathogenic organisms including; Enterococcus faecium ATCC 51299, Streptococcus mutans ATCC 35668, Shigella boydii ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Salmonella enteritidi PTCC, 1709, Candida albicans ATCC 10231 and Aspergillus fumigatus PTCC 5009 were investigated by the broth dilution method. Methanolic extract of six strains showed good activity amongst eight strains. Hexane extract, after methanolic extract has a good effect on the antimicrobial activity against five strains. All bacterial strains in this survey showed resistance against ethyl acetate and chloroformic extracts. S. glaucescens using four various solutions extracts against eight different human pathogens showed an important antimicrobial and antifungal activity. However, more investigation has to be done on separation, purification and detection of the active ingredients in order to recognize their antifungal and antifungal activity.

Salmonellosis as a zoonotic disease in dogs is not fully understood, and various reports have pointed to the transmission of antibiotic-resistant salmonella from dogs to humans. The current study aimed to evaluate the serologic and bacteriologic prevalence of Salmonella spp. in stray dogs placed in animal shelters around Tehran, compare the results to those of asymptomatic dogs, and determine the serotype of isolated species, as well as their antibiotic susceptibility pattern. A total of 100 fecal swab and blood samples were obtained from symptomatic and apparently healthy dogs (clinically) placed in four animal shelters around Tehran, Iran. Fecal and blood culture, as well as dog food culture, tube agglutination test, serotyping, and antibiotic susceptibility testing were performed on the samples. Fever, lethargy, diarrhea, and abdominal pain were observed in all the dogs in the case group, and bloody diarrhea was the least commonly detected symptom in clinical examination. A number of 11 and 4 collected fecal swabs from the case and control groups were positive for Salmonella spp., respectively. The polymerase chain reaction (PCR) also confirmed the laboratory tests results. Blood culture on the selective medium was negative for all the cases. Moreover, 60% and 100% of dogs in the case and control groups showed inflammatory markers in their blood test. The tube agglutination test was positive for 12% of the samples from the case group, while it was positive only for 5% of cases in the control group. The highest and lowest antibiotic resistance was observed against gentamicin and ciprofloxacin from the case group, respectively. Salmonella spp. infection in stray dogs placed in animal shelters is a great public health concern. In this regard, it is recommended that these animals be regularly monitored since they serve as Salmonella carriers.

  Multiple Sclerosis is a vascular disease in the central nervous system. There is evidence that Clostridium perfringens epsilon toxin causes damage to the myelin of the neuronal cells by increasing the permeability of the blood-brain barrier, and therefore contributes to the disease. Therefore, the aim of this study was the molecular identification of C. perfringens Epsilon toxin in samples isolated from multiple sclerosis patients using multiplex-PCR.

In this descriptive cross-sectional study, 60 stool samples of patients with multiple sclerosis and 60 specimens of healthy individuals were studied. e-toxin producing C. perfringens infection was examined by the M-PCR method after genomic DNA extraction.

  of 60 fecal specimens obtained from patients with multiple sclerosis, 11 (18.3%) and 2 (3.3%) samples were positive for 16S rRNA and ε-toxin-encoding gene, respectively.

The results of this study showed that the prevalence of C. perfringens toxigenic strains in MS patients are higher than in the control group, which can indicate the association between the presence of this toxin and MS disease.

Approximately three-fourths of women experience an episode of vaginal candidiasis. One of the virulence factors, is the ability of attachment of C. albicans to the host tissue through factors such as sticky molecules and proteins that accelerate this connection. The aim of this study was to investigate the frequency of biofilm formation genes in C. albicans strains isolated from vaginosis and to compare the expression of als gene in strains treated with iron nanoparticles in the in vitro using real time -PCR. In this descriptive cross-sectional study conducted in Shariati Hospital during a 6-months period, 100 isolates Candidafrom vaginal specimens were evaluated. After identification of C. albicans strains using standard biochemical and microbiological tests, genomic DNA extraction was performed using the CinnaGen Company Kit. Multiplex-PCR assays were performed to detect als, hwp1 and hyr genes. Also, to investigate the effect of iron nanoparticle on expression of als gene, RNA, all isolates containing this gene were extracted using Cinnapure kit. After cDNA synthesis, real time PCR test was performed. Molecular analysis results showed that from the total of 60 isolates of C. albicans, 34 isolates (56.66%) had the hwp gene, 46 isolates (76.66%) had Als gene and 33 isolates (55%) had Hyr gene. The results of the asl melting curve in clinical strains of C. albicans and treated with iron nanoparticles showed that the main peak for this gene occurs at 85.34 ° C. Als expression in the treated isolates was lower than untreated, which indicates the positive role of the treatment in reducing Als gene expression in these isolates.

  Candida albicans are human selective symbiosis, mainly located in the gastrointestinal tract. This group of fungi is pathogenic where host resistance reduces to local or systemic infection. Bacteria are the most common cause of vaginitis, but candida albicans are the second leading cause of such infections. Candida albicans are responsible for 70-90% of the fungal infections in the vaginal area. To study the genomic polymorphism of Candida albicans isolated from women with vaginitis using RAPD-PCR method. This cross-sectional study was designed and implemented by descriptive-analytic method. In this research, 60 samples of 30 vaginal samples from Shariati Hospital (Tehran) and 30 samples from Kerman Women's Hospital were collected by gynecologist and obstetrician. Using a sterile loop, various species yeasts were cultured on a differential chromatographed medium of Candida agar. Differentiation of subspecies and study of the possible relationship between specific strains and clinical forms was done. Typing of Albicans isolates was also done using RAPD-PCR method with random primers. Increased non-albicans and resistance to drugs not only in different parts of Iran but also in the world have been reported. In this study, based on the results of culture and direct experiment, 60 people were found to be infected with candida volvaginitis infection in two provinces of Kerman and Tehran, which were genetically similar and different in 14 clusters. Some clusters have repeated strains indicating similarity to genomic strains. There are several methods for genomic fingerprinting of different types of clinical isolates of candida albicans, using Pulsed Field Gel Electrophoresis (Pulsed Field Gel Electrophoresis) technique, fingerprinting methods based on ribotaiping, and fingerprinting methods based on PCR for epidemiological outbreaks are useful.
Conflict of interest: non declared

 Helicobacter pylori is a gram negative, curved, oxidase-fermented fermentation agent. This organism has been identified as a typical bacterial strain of cancer. The bacteria are the main cause of many gastrointestinal diseases such as stomach inflammation, duodenal ulcer, gastric ulcer, duodenal ulcer, and in particular gastric cancer.  A total of 120 patients with periodontitis referring to Tehran dental clinics were selected from 111 dental plaque samples. Sterile physiology serum was used to transfer the sample. Growth in Brucella agar + 1% starch + 10-7% at 37 ° C and 95% humidity and CO2 concentration of 0.5% in CO2 incubator. In order to identify the molecules, DNA extraction was performed and then the sequences of JW22 and JW23 regions were used for accurate identification. The molecular analysis of the studied genes showed that the prevalence of cagA, cagT, cagE, vacA and hrgA genes showed that the prevalence of these genes was 19% of Helicobacter pylori strains of 3 (57.5%), 1 (0/19%), 2 (34/0%), 1 (19/0%) and 1 (19/0%) respectively. The multiplex PCR method used in this study was able to detect Helicobacter pylori genotypes based on virulence genes. According to the findings of this study, the hrgA genotype and all types of cagA motifs can predict the chronicity of the disease and use the antibiotic resistance pattern appropriate for the design of appropriate treatment protocol, the drug was used to eradicate the disease and prevented the infection from entering the chronic phase.

Quality of care is one of the basic rights of patients. Since the survey of nurses and patients' viewpoint can provide more comprehensive information about the status of provision of services, this study aimed to assess the quality of nursing care from nurses and patients viewpoints. This applied descriptive-analytic study was conducted in Zanjan in 2016. In this study, 150 patients and 16 nurses working in the only dialysis center in Zanjan located in Valiasr Hospital were enrolled by census method. Data were completed using the SERVQUAL questionnaire (Service Quality). Paired t-test, independent t-test and ANOVA and SPSS 16 software were used to analyze the data. From the perspective of the patients participating in the study, the gap (i.e. the difference in what was expected of the patients and what they understood in practice) was in the quality of providing services in all aspects of perceptions and expectations, except for the guarantee of existence (P < 0.001). From the viewpoint of nurses, there was a gap in the quality of services in all aspects of perceptions and expectations (P < 0.001). There was a significant relationship between education (P = 0.027) and occupation (P = 0.002). The results of this study indicate that there is a gap in the quality of nursing care in the dimensions of perceptions and expectations of patients and nurses. Therefore, it is recommended to design and implement programs to improve the quality of nursing services after investigating and identifying the reasons for the lack of quality of services

In recent years, biological control of parasites by essential oils (EOs) derived from plants is one of the alternatives to synthetic pesticides.Melissa officinalis from Lamiaceae family is distributed in many parts of Iran. It is known as an excellent source of antioxidants, antibacterial, antiviral and antifungal constituents. The present study investigated the insecticide properties of M. officinalis against Tetranychus urticae tick.

The EO of different parts of plant was extracted and analyzed by gas chromatography and mass spectrometry (GC/MS). The ticks were placed on the filter paper in the bottom of a petri dish (9 mm), and contact toxicity assay was then performed by contacting the extract with the ticks.

The EO of leaves showed the most potent insecticidal effect while the stem EO demonstrated the weakest effect. The lowest concentration of EO from the leaves showed more considerable insecticide activity compared to the highest concentration of stem and flower EOs.

Melissa officinalis is an effective insecticide with potent effect against T. urticae and it could be suggested as a natural pesticide against T. urticae.

In spring of 2012,two ewes of a sheep flock from west region of Alborz province with neurological disorders as circling,dullness,depression,incoordination and anorexia  were referred to veterinary school hospital of Islamic Azad University of Karaj branch.on initial examination,cantral nervous system lesions were suspected.in order to confirm the diagnosis,one of them utanaized.macroscopic findings in necropsy were leptomeningial opacity,cloudy cerebrospinal fluid and meningeal vessels congestion.many foci of yellow-brown discoloration (0.1 mm in diameter) were seen in brain stem section.microscopically,neuronal necrosis,gliosis,perivascular cuffing,neutrophilic micro abscesses and meningoencephalitis were observed.listeria monocytogenes was isolated from brain samples and CSF in bacteriological Lab.on the basis of macroscopic signs,histopathological findings and microbiological test results,meningoencephalic listeriosis was detected in these cases.