Study of Genetic Diversity of Wheat Genotypes by RAPD Markers

The genetic diversity of 48 wheat genotypes was studied by RAPD markers at 2012 in laboratory of Department of Agronomy and Plant Breeding، University of Mohaghegh Ardebili. 10 primers from 70 used primers showed suitable banding patterns. These primers totally produced 73 polymorphic bands with mean 7. 3 for each primer. The number of bands for 55 and 59 primers were 5 and 11، respectively. The total numbers of polymorphic bands within varieties verified between 7-57 bands that mean of bands number were 0. 7 for ‘Azadi’ and 5. 7 for ‘Ghods’. There was remarkable diversity among varieties based on Nei’s gene variation index. The primers 30 and 18 had the highest (0. 408) and lowest (0. 226) genetic diversity، respectively. The cluster analysis on based of Nei’s distance and UPGMA method، distinguished four different groups. In this grouping، external varsities lied separately in second group. Thus، RAPD marker was successful in separating external from internal varsities. Based on principal coordinates analysis three coordinates accounted for only 61. 7 percent of variation which indicated the proper distribution of markers across wheat genome under study because of relatively low percentage determined by the three first coordinates.