Determining the Frequency of Panton-Valentine Leukocidin (PVL Genes), and Collagen-Binding Protein (cna) in Staphylococcus Aureus Strains Isolated from Clinical Specimens and Antibiotic Resistance: A Short Report

Panton-Valentine leukocidin (PVL) toxin is a hemolytic exotoxin that increases the permeability of the cell membrane, thus causing lysis of leukocytes and tissue necrosis. Collagen-binding protein (Cna) adhesion Staphylococcus aureus is responsible for binding to collagen and the major virulence factor in arthritis and osteomyelitis. The purpose of this study was to investigate the presence of antibiotic resistance and virulence genes for PVL, Cna in Staphylococcus aureus strains isolated from clinical specimens by Multiplex PCR. In this cross-sectional, descriptive study, 67 samples of Staphylococcus aureus were isolated. Based on CLSI antimicrobial susceptibility guidline, different antibiotics were tested. Multiplex PCR assay was performed. Finndings showed that the susceptibility to vancomycin and linezolid antibiotics was 97.1% and maximum resistance is to clindamycin was 23.9%. the frequency of Cna gene in clinical samples was 41.7% while PVL genes were not detected in the samples. Because of the importance of methicillin resistance Staphylococcus aureus as the cause of infection among people, especially resistant hospital infections, due to increasing use of antibiotics, knowledge of the level of resistance to these agents is essential. Multiplex PCR is a simple, sensitive, inexpensive, relatively quick and very specific in addition, it has an ability to identify multiple genes simultaneously.