Application of Surface Plasmon Resonance (SPR)Sensors for Detectionof Vibrio cholerae

Surface plasmon resonance (SPR) sensors are an optical platform capable of highly sensitive and specific measuring of biomolecular interactions in real-time. This label-free technology can quantify the kinetics, affinity and concentration of surface interactions. SPRsensors have been used to detect bacterial pathogens in clinical and food-related samples. SPR-based immunosensors that coupled with a specific antigen-antibody reaction, have become a promising tool for the quantification of bacteria as it offers sensitive, specific, rapid, and label-free detection. In this paper, we evaluated application of a SPRbased immunosensor for detection of Vibrio cholera causative agent of cholera by a dual channel Surface plasmon resonance device. At first a Self Assembled Monolayer (SAM) of 11-Mercaptoundecanoic acid was formed on sensor chip and after that antibody againstVibrio cholera was immobilized on chip covalently. Then different dilutions of Vibrio cholera cultures was passed on immobilized antibody and the response of SPR was recorded. The specificity of this nanobiosensor was studied by interaction of some non Vibrio cholera bacteria.