Prevalence of Methicillin resistant (mecA) and Panton-Valentine leucocidin (pvl) genes among Staphylococcus aureus isolates recovered from clinical samples of Rasht hospitals

Staphylococcus aureus is one of the most important nosocomial infection agent and now methicillin-resistant S. aureus have spread. Also, panton-valentin leukocidin is one of important virulence factors in S. aureus. The study was aimed to evaluate the antibiotic resistant, frequency methicillin resistant and panton- valentin leukocidin genes in clinical S. aureus isolates from hospitals of Rasht, Iran.
This cross-sectional study was carried out on 250 clinical samples collected from hospitals of Rasht during one year (2013-2014). The S. aureus isolates were identified by microbiological methods. Antibiotic sensitivity was performed by CSLI method using disk diffusion method. In addition, the presence of methicillin resistant (mecA) and panton-valentin leukocidin (pvl) genes were evaluated using polymerase chain reaction (PCR).
Totally, 50 S. aureus isolates were recovered. The results of antibiotic susceptibility tests showed that 34 out of 50 S. aureus isolates (68%) were resistant to methicillin. In addition, the prevalence of mecA and pvl gene among isolates were 60% (30 isolates) and 20% (10 isolates), respectively.
This study showed increased resistance to different antibiotics in S. aureus that is a serious warning to the treatment of infections caused by this bacterium in the region.