جستجوی مقالات مرتبط با کلیدواژه « meca » در نشریات گروه « زیست شناسی »

Eugenol is the major constituents of clove oil, which is known as an anti-bacterial and anti-oxidant agent. Staphylococcus aureus is most common pathogens that cause nosocomial infections and some bacterial infections death. In this study, the effect of Eugenol on the expression of mecA gene and the interaction with mecA protein (PBP2a) was evaluated in methicillin resistant isolates of Staphylococcus aureus.

Antibacterial activity of Eugenol was investigated in isolates of Staphylococcus aureus with DISC diffusion, MIC and MBC. Interaction between Eugenol and mecA protein (PBP2) was evaluated with bioinformatic approaches. The expression of mecA gene was assessed in Eugenol treated and untreated isolates by Q-RT-PCR.

results of this study showed that Eugenol inhibited bacterial growth in the DISCs in 80% of Staphylococcus aureus isolates. MIC and MBC of Eugenol in the most isolates were determined 5.22 mg/ml and 10.44 mg/ml, respectively. In silico analysis showed interaction between Eugenol and mecA protein (PBP2). mecA gene downregulated in Eugenol treated isolates (5.22 mg/ml) in compared to control.

It seems that Eugenol led to growth inhibition of Staphylococcus aureus isolates through both interaction with PBP2 and mecA gene downregulation.

Staphylococcus aureus is one of the most important nosocomial infection agent and now methicillin-resistant S. aureus have spread. Also, panton-valentin leukocidin is one of important virulence factors in S. aureus. The study was aimed to evaluate the antibiotic resistant, frequency methicillin resistant and panton- valentin leukocidin genes in clinical S. aureus isolates from hospitals of Rasht, Iran.
This cross-sectional study was carried out on 250 clinical samples collected from hospitals of Rasht during one year (2013-2014). The S. aureus isolates were identified by microbiological methods. Antibiotic sensitivity was performed by CSLI method using disk diffusion method. In addition, the presence of methicillin resistant (mecA) and panton-valentin leukocidin (pvl) genes were evaluated using polymerase chain reaction (PCR).
Totally, 50 S. aureus isolates were recovered. The results of antibiotic susceptibility tests showed that 34 out of 50 S. aureus isolates (68%) were resistant to methicillin. In addition, the prevalence of mecA and pvl gene among isolates were 60% (30 isolates) and 20% (10 isolates), respectively.
This study showed increased resistance to different antibiotics in S. aureus that is a serious warning to the treatment of infections caused by this bacterium in the region.

Infectious diseases caused by Gram-positive bacteria Staphylococcus aureus strains that resistant to drugs، which have often a hospital source، is increasing in many countries. In this applied study، 40 persons of therapeutic Personnel including nurses، technicians، experts and patients from Zabol hospital and 40 students of Zabol University who were willing to participate in this research were selected. They were evaluated in terms of vectors. Samples، with separate sterile swabs were taken from the anterior portion of the nose and throat then were immediately transferred to the culture medium. Finally، by standardized tests، Staphylococcus aureus was detected. Then-PCR method، by using specific primers mecA، the Staphylococcus aureus was isolated. The results of this research showed that nasal carriers were 17/41 percent، throat carriers were 29/35 percent، nose/throat carriers were 76/11 and about 17/41 percent of all Staphylococcus aureus isolated، were resistant to methicillin. Due to the high rate of carriers of Staphylococcus aureus that resistant to methicillin، for Identifying these people and treat them in order to eradicate the infectious agent، can be one of the important ways to prevent hospital infections that in terms of the health and decreasing mortality، social، economic and health impacts is very important.