Cancer cell detection using electrochemical nanobiosensor based on graphene / gold nanoparticle
Author(s):
Abstract:
Introduction
Nowadays, early cancer detection and effective treatment is crucial for improved prognosis and cancer management. In particular, the accurate qualitative detection of cancer cells represents a critical step in cancer diagnosis. The aim of this study was to examine Cancer cell detection using electrochemical nanobiosensor based on graphene / gold nanoparticle.Materials And Methods
Modified graphene oxide/gold nanoparticle electrodes were employed to increase the sensitivity of human breast cancer MCF-7 cells detection, using CD44 biomarker. Frist the electrodes were modified with graphene, then gold nanoparticles were sediment on graphene-modified electrode. Then CD44 monoclonal antibody conjugated on the surface of gold nanoparticles, on graphene-modified electrode. Finally, the performance of the fabricated biosensors were investigated by using a common reference electrode composed of silver-silver chloride and a common platinum counter electrode at different antigen concentrations with the buffer and serum.Results
The proposed electrochemical cytosensor delivered a high sensitivity with the average of 1.12 μA / cells ml-1, and a low detection limit of 6 cells.Conclusion
These results indicate that the cytosensor has great potential in diagnosis of cancersKeywords:
Language:
Persian
Published:
Koomesh, Volume:18 Issue: 1, 2016
Pages:
211 to 219
https://magiran.com/p1587794
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