Effect of artificial collapse of mouse blastocyst on viability and Wnt3a gene expression

In recent years, using the assisted hatching techniques such as puncture of blastocyst, are used in in-vitro fertilization (IVF) for increase the rate of implantation. This study was designed in order to evaluate of AC effect in survival and hatching rate of mouse blastocysts by expression of Wnt3a gene. In vivo and In vitro blastocysts are obtained. Some of them in in-vivo and In vitro groups were collapsed with a micro-needle and non-collapsed blastocysts in each group were used as a control. Evaluated survival and hatching rate and quantitative expression of Wnt3a gene in both groups was investigated by using Real time PCR. Our results revealed that AC of blastocyst in in-vivo and In vitro blastocysts did not improve survival rate although hatching rate had a significant difference in the collapsed blastocysts compared to non-collapsed blastocysts in both groups. The results real time PCR quantitative analysis showed that expression level of Wnt3a gene has increased in in-vivo blastocysts and it has decreased in in-vitro blastocysts comparing to control group in both treatment. AC could be effective way to improve the assisted hatching techniques.