Histopathological effect of organic pollutant on male and female gonads of Goldfish (Carassius auratus)

Today, the reproductive systems of fish as a valuable model to study the effects of the significant components of the vile endocrine disruption have been considered. This study was inspected the testis and ovary histopathological change in Carassius auratus (Goldfish) as a suitable alive model, exposed to two common pollutants Naphthalene, Butachlor. The fishes received Naphthalene, Butachlor (with doses of 200 μg/l, 0.28 μg/l respectively) during 15 days including control treatment (without the chemicals). At the end of experiment gonad tissue was examined after fixed by fixative, processed, and embedded in paraffin as sectioned at 6μ thickness. Further stained by Hematoxylin- Eosin general method. Histopathological lesions observed in the testes included degenerated germ cells, proliferation and hypertrophy of Sertoli cells, development of connective tissue, fibrosis and inhibited development of lobules, filled seminiferous tubules with fertile sperm (at Naphtalene treatment), seminiferous tubules with no fertile sperm in it (at Butachlor treatments). These markers weren’t observed in control group and indicate that environmental contaminants can disrupt reproductive system of the aquatic animals. Histopathological lesions observed in the ovary included degenerated ooytes, cytoplasmic retraction, adhesion of the oocytes, karyoplasmic clumping and its retraction within the oocytes and anucleated oocytes. In the ovary of control treatment, most of oocytes were at vitellogenesis stage, but in Naphthalene treatment the majority of the oocytes observed at previtellogenesis. In general it can be concluded that endocrine disruption has ability to change various stages of gonadal development and gamete development stages of males and females fish.