Effects of Cholesterol loaded cyclodextrin on vitrification of the cat epididymal sperm

Cholesterol loaded cyclodextrin (CLC) efficiently protected sperm of some species against cryodamage. On the other hand, vitrification of sperm especially in human opened a new horizon in the field of sperm freezing. The aim of the present study was to evaluate the effect of CLC on vitrification of cat sperm. The tom epididymal sperm (n=22 pairs) was extracted from epididymides and subjected to vitrification in TRIS based solution that was incorporated with or without CLC. The warmed sperm was analyzed for motility, viability and morphology. Vitrification significantly (P