The effect of eight weeks high intensity interval training on plasma irisin and subcutaneous adipose tissue UCP-1in male obese rats

Phenotype changes of adipose tissue due to exercise is a new theory which has recently been proposed and Irisin hormone which was discovered in 2012 has an important role in this process. However, identification of its molecular mechanism is being investigated. The aim of this study is to investigate the effect of high intensity interval training on plasma Irisin and insulin resistance and subcutaneous adipose tissue UCP-1 in male obese rats.

In this study, 14 obese rats with high-fat diet were divided to 2 groups: high intensity interval training group (n=7) and control group (n= 7). The training protocol included 8 weeks of high intensity interval training 5 times a week and in the form of training bouts with maximum speed of 80-85% intensity for 2 minutes and 1-minute active­­ ­rest periods that started from 6 training bouts in the first week and extended to 12 training bouts in the last week. ELISA method was used to measure plasma irisin, insulin and glucose and the UCP-1 subcutaneous fat. Independent t-tests were used for data analysis and the significance level was considered at P≤0/05.

Data analysis showed that plasma Irisin and UCP-1 Subcutaneous fat significantly increased due to 8 weeks high- intense interval training (P=0/01, P=0/04) and also insulin resistance index had significant decrease (P=0/01) in the experimental group compared to the control group.

Altogether, current study shows that consuming one dose of caffeine or caffeine-carbohydrate did not effect on lipid metabolism and aerobic function.