Molecular study of the blaZ Staphylococcus aureus gene isolated from clinical samples

Bacterial resistants to beta-lactam antibiotics, are steadily expanding. Excessive consumption beta-lactam antibiotics in the treatment of related Staphylococcus aureus infections caused the emergence of beta-lactamase enzymes in strains. Identification and analysis of molecular characteristics of these strains can be effective to select on the appropriate antibiotic treatment.
Initially, the isolates collected from clinical samples were screened by biochemical tests. After the whole Staphylococcus aureus resistance to beta-lactam antibiotics, were evaluated by used of molecular-specific primers.
Of 496 isolates obtained from different clinical samples, 147 isolates were identified as S. aureus. The highest resistance to the antibiotics had related to penicillin, Oxacillin, Cefocithin and the lowest resistance antibiotics had related to Vancomycin. From the 147 samples, 143 samples were blaZ genes that allocated to over 97 percent.
According to the results of molecular tests, in the Zahedan spread of carrier-lactamase strains is very high. This would be to treat all staphylococcal infections of beta lactam antibiotics are not used as much as possible to the access to early treatment prevented the increase in resistant strains.