Essential oil composition and antimicrobial activity of Acinos graveolense (M.B.) Link. extract from Ghamsar, Kashan

Acinos Miller. is belongs to Lamiaceae family, which grows as bush-herbaceous and consists of more than 10 species; two spaces from Aonos has been reported in the flora of Iran. In this study the essential oil composition and antibacterial activity of Acinos graveolense (M.B.) Link extract has been studied. The aerial parts of the plant in blooming were collected from natural habitats (Ghamsar area), in spring 2014. It was dried in laboratory conditions, the essential oil were isolated by simultaneous steam distillation extraction (SDE) and were analysed by using GC and GC-MS. The methanol extract was obtained by maceration method and the antimicrobial activity of plant extract was measured by disk diffusion and minimum inhibitory concentration against eleven microorganisms. The yield of the oil was 0.1% w/w. the analysis led to identification of 27 compounds. The main essential oil components were germacrene-D (56.6%), bicyclogermacrene (8.7%), caryophyllene oxide (7.5%), e-caryophyllene (4.5%), and limonene (3.4%). In addition, sesquiterpens hydrocarbon was the main component of the oil. The extract of Acinos graveolense against three tested bacteria (Klebsiella pneumonia, Staphylococcusepidermidis and Salmonella paratyphi-Aserotype) were showed low antimicrobial activity. In conclusion, due to the high amounts of sesquiterpenes in this essential oil and its antimicrobial activity, we could reports that the aerial part of A. graveolense is suitable for the preparation of natural antimicrobial agent.