نشریه اکوفیتو شیمی گیاهان دارویی
سال دوازدهم شماره 1 (پیاپی 45، بهار 1403)

Organic manuares have important effectiveness on quantity and quality of medicinal plants and this plants have important role in food industries, then this research was done to evaluate the effects of organic and chemical fertilizers on essential oil content and composition of Thyme. This research was conducted in a randomized complete block design with 4 replications in Research Farm of Islamic Azad University Branch of Shahrekord at 2020 and 2021. Treatments were: control (no fertilizer), cow manure, sheep manure, hen manure, horse manure, vermi-compost and complete chemical fertilizers. Each of animal manuares and vermi-compost in 20 t.ha-1 in planting time and complete chemical fertilizer in 1.5 g.l-1 concentration after V10 stage in two step by 3 weeks interval, were used. Shoot of plant samples beginning of flowering stage in end of vegetative stage (252-270 leaf) prepared from treatments separately in the end of august. For measurement of the content and yield of the volatile oil, 1000g of the fresh shoots harvested and mixed with water and placed in a flask connected to the condenser of a Clevenger apparatus and the GC analysis was done to detect the essential oil composition. The most weight of dry matter (352±17.1 g.m-2 in first year and 359.04±18.9 g.m-2 in second year) and essential oil content (0.91±0.1% in first year and 1.1±0.01% in second year) were observed in chemical fertilizer was the same group with vermi-compost and hen manure. Four components of P-cymene, Gamma terpinen, Carvacrol and Thymyl acetate are classified as cyclic monoterpene made more than 70% of essential oil content in different treatments. 29 components in essential oil of treatments were detected. The greatest amount of major components were Carvacrol (35.2-36.8%) in chemical fertilizer and vermicompost, P-cymene (8.3-7.8%) in chemical fertilizer; Gamma terpinene (18.6-18.7%) in vermi-compost and Thymyl acetate (4.6-4.8%) in hen manure.

The genus Lepidium from the family Brassicaceae has 16 species in Iran, and the most famous species is garden cress (L. sativum), which is used as a vegetable or medicinal plant. The purpose of this study is to evaluate the quality and quantity of seed essential oil of seven L. sativum populations collected from different climatic regions of Iran. Seeds of L. sativum populations were hydro-distillated by clevenger, and the chemical composition of the essential oils and their quantitative percentages were identified using gas chromatography-mass spectrometer (GC-MS). The main compounds of seed essential oil were monoterpenes 1,8-cineole (44.5%), α-terpinyl acetate (13.9%) and α-pinene (7.7%). The populations of Karaj, Kerman and Qazvin had the highest amounts of 1,8-cineole, α-terpinyl acetate and α-pinene, respectively. The results of cluster analysis based on the chemical composition of garden cress seed essential oil using the Nearest neighbor method showed that seven studied populations were divided into two groups, and this grouping did not match the geographical coordinates of the regions. The first group included the populations of Kerman, Qazvin, Shahriar and Tabriz, and the second group were the populations of Shiraz, Hamadan and Karaj, which had the highest amount of 1,8-cineole and the lowest amount of α-terpinyl acetate and 7,10-hexadecadienoic acid, methyl ester. According to the results of the bi-plot diagram of principle component analysis (PCA), Karaj population -which had the lowest values of the first and second components- was placed in a distinct group. No significant correlation was observed between the main compositions of the essential oil with the geographic coordinates and weather conditions of the collection sites.

Ethnobotany, as a branch of indigenous knowledge, investigates how people of a tribe or community use the plants of a region. Despite the huge advances in the production of chemical drugs, the use of herbal and traditional drugs still seems to be efficient and irreplaceable. The purpose of this research is to know the medicinal plants used by the people in Chaharcheshmeh village of Markazi province. For this purpose, the floristic list of the target area was first prepared using the field survey method, and then, using questionnaires, the area’s local residents’ local knowledge such as the local name of the plant, the organ used, the method of consumption, and the use of the medicinal plants of the area was obtained through open, semi-structured and collaborative interview methods with native people. According to the results of this research, 41 plant species belonging to 17 families are used by the people of Chahar Cheshme village. Among them, Lamiaceae, Fabaceae, Asteraceae and Apiaceae families are the most important. The highest Relative Frequency Citaion indices (RFC) of the records belong to mountain tea (0.85), oregano (0.76), thyme (0.75), and mint (0.72), respectively. The most common uses of these medicinal plants were to eliminate digestive and respiratory disorders. The people of this region use these medicinal plants mostly in the form of decoction, sweating, topical application, and powder. Based on the results of this research, it can be concluded that utilizing the indigenous knowledge of the residents of Chahar Cheshmeh village can contribute to enhancing the awareness of contemporary individuals in the field of herbal medicine utilization as an alternative to chemical drugs. Furthermore, these natural compounds can be utilized in the pharmaceutical industry to mitigate the side effects caused by chemical substances.

This study aimed to investigate the phytochemical composition, antioxidant activity, and anticancer effects of root and sprout samples from Multi-hued Bulk, Black, and Titicaca Quinoa varieties (Chenopodium quinoa willd.) on Human Gastric Adenocarcinoma Cell Line (AGS). These varieties were cultivated using a complete randomized block design with four replications in arid and semi-arid regions of Iran (research field of Yazd University). Root samples were harvested before seed filling from a soil depth of 30 cm. Once the seed germination and filling stages were complete, 100 g of seeds from each variety were randomly selected without hulling to produce sprouts. The highest saponin contents (mg/g dry weight) were observed in the Black root (24.4), followed by the Titicaca root (7.1). The lowest saponin content (0.6) was found in the Black sprout. The phytochemical composition, and antioxidant activity of the hydroethanolic (80:20) extract (obtained using the Soxhlet method), at 100, 250, 500, and 1000 mg/mL were evaluated for root and sprout samples of all three varieties. Based on the results, the Black and Titicaca sprout extract had significantly higher total phenolic (172.68 mgGAE/gDW) and flavonoid (128.17 mgQ/gDW) contents, as well as antioxidant capacity at 1000 mg/mL compared to the corresponding samples from the Multi-hued Bulk variety and the root samples of all three varieties. The lowest and highest DPPH IC50 value was observed in the Black sprout and Multi-hued Bulk root extracts (1.11 and 3.15 mg/mL, respictively). The average cell viability of AGS was observed in the Black sprout extract (33.46) and Titicaca (36.11) varieties at 1000 mg/mL, 72 hours after the treatment, respectively. Findings suggested that the phytochemical and antioxidant properties of Quinoa sprouts, compared to hulled and unhulled seeds of the investigated varieties, could contribute to the development and accessibility of this medicinal plant for patients with cancer.

The purpose of this study was to evaluate the performance of safflower under the influence of humic acid and zinc chelate in field conditions. The experiment was carried out in a factorial form in the form of randomized complete blocks in a farm in Saqez city in 2019. The results showed that the effect of humic acid treatment was significant at the 5% probability level on plant height and on the number of sub-branches at the 5% probability level. The effect of zinc chelate and the interaction of treatments on both traits were significant at the 1% probability level. The use of all three foliar treatments (humic acid zinc chelate and the interaction of treatments) had a significant effect on the number of seeds per plant. Comparison of averages showed. The highest number of seeds per plant in the combined treatment of 1 mg/liter of zinc chelate and 400 mg/liter of humic acid (125/33) as well as the treatment of 2 mg/liter of zinc chelate (123/25) and the lowest number in There was a combined treatment of 100 mg/l of humic acid and 2 mg/l of zinc chelate. The results showed that zinc chelate treatment and the combined treatment of zinc chelate and humic acid had a significant effect on the number of leaves in safflower plant, but humic acid treatment did not have a significant effect on this trait. C The results of the present study showed that the amount of linoleic acid increased with the addition of humic acid and zinc chelate, but the amount of oleic acid decreased.

In this article, a phytochemical investigation including identification and measurement of some phenolic compounds in the hydroalcoholic extracts of the Cichorium intybus L. collected from 6 localities was carried out. Among the studied compounds, chicoric acid (0.55 to 6.04 mg per gram of dry plant), chlorogenic acid (0.50 to 5.37 mg per gram of dry plant), and rutin (0.56 to 2.25 mg per gram of dry plant) were found in extracts of aerial parts and chicoric acid (0.03% to 0.29 mg per gram of dry plant) and chlorogenic acid (0.84% to 2.88 mg per gram of dry plant) were found in the roots of all samples. Among the studied populations, the highest amount of major compounds (chicoric acid and chlorogenic acid) were observed in the aerial parts of the sample collected from the National Botanical Garden of Iran with 6.04 mg of chicoric acid and 5.37 mg chlorogenic acid per gram of dry plant.

Ferula gummosa (Barijeh) belongs to the Apiaceae family and is one of the most important pasture, medicinal and industrial plants, whose oleo-gum-resin (galbanum) has a commercial aspect. The essential oil and oleoresin of F. gummosa are widely used in the food, pharmaceutical and cosmetic industries. The aim of the present study is to investigate and compare the physicochemical properties of the essential oil obtained from the oleo-gum-resin of two F. gummosa populations from Isfahan and Fars habitats. For this purpose, the oleo-gum-resin of F. gummosa was collected from two areas in Kashan and Fars. The essential oil was then extracted from the fresh oleo-gum-resin by water and steam distillation. The essential oil was analyzed quantitatively and qualitatively using GC and GC/MS devices. The appearance, physical and chemical characteristics of the essential oil of two populations of F. gummosa, including relative density, refractive index, optical rotation, and phytochemicals of the essential oil were investigated and compared. Based on the results, the essential oil of F. gummosa is a transparent and colorless liquid with a turpentine odour and a distinct green note. The essential oil of two populations of Kashan and Fars has relative density of 0.874 and 0.869, refractive index of 1.4810 and 1.4795, and optical rotation of 11.35° and 23.52°, respectively. The results of comparing the average physicochemical characteristics of the essential oil obtained from the oleo-gum-resin of the two F. gummosa populations from Fars and Kashan indicate that there is no significant difference in the traits of relative density and refractive index, and on the other hand, there is a significant difference in the amount of optical rotation of the two essential oil samples. Based on the essential oil analysis, hydrocarbon monoterpenes including β-pinene (71% and 68.8%), δ-3-carne (9.4% and 5.2%), myrcene (3.9% and 2.4%), α-pinene (3.6% and 3.4%) and limonene (2.4% and 15.6%) were identified as the main compounds of essential oil in two F. gummosa populations from Kashan and Fars, respectively.

This research aimed to determine the diversity and identify some biochemical parameters among different garlic cultivars. A factorial experiment with a completely randomized design and three replications was carried out in the central laboratory of Razi University in 2024 to group six ecotypes from different parts of Hamedan province based on their place of growth and determine their biochemical traits. The results of variance analysis of the studied traits showed a significant difference between the ecotypes in the traits of total chlorophyll, chlorophyll a, chlorophyll b, soluble sugar, flavonoid, and the two leaf and seed samples. The results of examining the interaction effect of ecotype in the samples indicated the significance of the total phenol content index among ecotypes. The ecotypes were grouped based on the cluster diagram and decomposition into main components, and they were placed in similar groups in terms of their biochemical traits. Considering the geographical conditions of the samples and their presence in the same geographic latitudes, the difference between the samples may be attributed to the difference between the genotypes of the ecotypes. There was a positive and significant correlation between chlorophyll a, chlorophyll b, and total chlorophyll. There was also a positive and significant correlation between phenol and flavonoid content. Based on the traits studied, Asad-abad and then Bahar-2 ecotypes can be introduced as desirable examples in clinical studies and can also be used for breeding programs.