Spawning induction in Anzeh (Barbus Esocinus) by injection LHRH-A2 with Pituitary Gland Extract

Anzeh Fish (Barbus esocinus) is one of the species is belonging to family Cyprinidae and Genus Barbus and it is a domestic fish of Khoozestan Province. Considering to internal water resources of the province, overfishing of domestic fish, natural resources of this species has been decreased. This study was conducted to achieve an applicable method in artificial propagation of Anzeh fish. So, 20 Anzeh female brood stocks with mean weight 11.43 kg were randomly selected and distributed among 3 treatment groups and one control group. 4, 7 and 10 µg/kg LHRH-A2 hormone (as preliminary injection) was injected in pectoral area respectively for treatment 1 to 3. 0.5 mg/kg Pituitary Gland Extract (PGE) for second injection and 4.5 mg/kg PGE for final injection. In control group, PGE was only injected in two stages, 0.5 mg/kg for first injection and 4.5 mg/kg for final injection. After incubation period, eggs were collected and fertilization was performed by dry method. Finally, reproduction indexes such as brood stocks response rate, working fecundity, fertilization rate, hatching rate were measured and studied. Based on results, the best reproduction indexes were seen in treatment 2 so that all fish responded to the injection and the reproduction indexes were as follows: response rate: 100%, working fecundity 257240±33658, fertilization rate 96.3±0.47, hatching rate 97±0.67, larval rate: 240400±15315 which were significantly different with other groups (P<0.05). Based on the results obtained from this study, three stages injection method by administration of 7 µg/kg LHRH-A2 hormone (as preliminary injection) and two injection of PGE ) for second injection and 4.5 mg/kg PGE can be used for successful artificial propagation of this domestic fish.