Effect of some injectable vitamins and trace minerals on blood antioxidant capacity and incidence of metabolic disorders in transition dairy cows

In this experiment, the effect of vitamin E (VE) and selenium (Se) combination and vitamin B12 (VB12) and iron (Fe) combination injection investigated on antioxidant status and health of transition dairy cows. Twenty heifers and 20 multiparous Holstein cows were divided to 4 balanced groups accoding to parity and BW. Experimental treatments consisted of 1) injection of 7 ml of NaCl % 0.9 (Control), 2) injection of 60 ml of VE and Se, 3) injection of 7 ml of VB12 and Fe and 4) injection of 60 ml of VE and Se with 7 ml VB12 and Fe. Solutions injectied on 21 and 7 day before calving and 7 d postpartum. Injection of VE and Se increased serum VE concentration and total antioxidant capacity (TAC) at calving day and day 7 and 14 postpartum, serum Se concentration and glutathione peroxidase activity at calving day and 14 day postpartum and blood neutrophils counts at calving day. Serum VB12 concentration at all sampling times, serum Fe concentration and catalase activity at calving day and serum TAC at calving day and day 7 and 14 postpartum were higher in cows received VB12 and Fe combination. The greatest incidence of retained placenta and mastitis were observed in control group. Overally, although, simultaneous injection of VE, Se, VB12 and Fe had no significant effectat on antioxidant status of transition dairy cows, but there is no observation of retained placenta and mastitis incidence in this group.