Effect of acute and high- intensity interval training period in normal and hot conditions on serum levels of glutathione peroxidase and HSP72 in active men

Anti oxidative enzymes and heat shock protein72 (HSP72) are two important internal body protective factors. The purpose of present study was to determine the effects of 1 and 12 sessions of high intensity interval training in warm and normal environment on serum levels of Glutathione peroxidase and HSP72 in active men (at least 8 hours per week). Twenty four physical education students of Kharazmi university were divided into three equal and homogenous groups include: exercise in warm environment(temperature 40± 1, humidity 35 ± 5),exercise in normal environment(temperature 24, humidity 35 ±5) and control group. Training protocol included running on treadmill with 85% vVO2max intensity in 5 sets per 150 second. Training sets separated from each others by running with 50% vVO2max intensity as a recovery time (150 second). Blood samples were taken before and after first activity session and 24 hours after last training session and Glutathione peroxidase (GPX) and HSP72 levels measured via ELISA method.
Data was analyzed using the independent and dependent t tests and one way ANOVA at significance level of p<0.05. Serum levels of GPX and HSP72 increased significantly after 1 and 12 sessions of high intensity interval training compared to pre-test in both of warm and normal environment groups. However any between group significant differences was not observed in these two groups . Furthermore, after 12 session of practice the serum level of GPX in both warm and normal environment training groups increased significantly in comparison with control group, but the serum level of HSP72 was not significant in two training groups when compared with control group. In general result of present study showed that 1 session and 1 high intensity interval training period is stimulator of GPX and HSP72 and arising the environmental temperature has no effect on response and adaptation values of mentioned factors.