Antibacterial effect of Zataria multiflora Essential oil against biofilms of Staphylococcus aureus Clinical Isolates

Antibiotic resistance is documented to be a serious problem that affects the choice of appropriate antibiotic therapy. One of the proposed methods to cope with biofilm bacteria is the use of alternative antibacterial treatments, which include natural antimicrobial substances such as plant essential oils. The aim of this study was to investigate the antimicrobial activity of Zataria multiflora essential oil against biofilms of Staphylococcus aureus clinical isolates. 10 Clinical isolates of S. aureus from various specimens having ability of biofilm formation were screen in this study, On the basis of their ability to attach to polymeric surfaces. Minimum Biofilm inhibitory concentration of Z. multiflora essential oil was estimated on polystyrene microtiter plates. The results indicated that 6 isolates of S. aureus included in this study were able to produce a stable biofilm and Z. multiflora essential oil had antibiofilm activities. Our study identifies that essential oil can be effectively used to control of S. aureus biofilms that Indicating the importance of natural agents as potential antibiofilm and antimicrobial agents.