Considering the anti-inflammatory and antioxidant activities of DDW and Mentha longifolia E.O on pulmonary damage induced by CLP

Sepsis is a major cause of morbidity and mortality in patients in the intensive care unit. Despite new supportive treatments and administration of high potent antibiotics, sepsis is overwhelmingly one of the risky factors in patient’s life. So, the purpose of this study is to investigate the effects of Deuterium Depleted Water (DDW) and M. longifolia essential oils (E.Os) on septic rats induced by CLP (Cecal Ligation and Puncture). Therefore, rats were divided into 7 groups: negative control (Laparotomy), CLP, treatment groups with DDW (15and 30 ppm), adjuvant with M. longifolia E.O (100 mg/kg b.w) and positive control group (indomethacin). Then, 24h after CLP induction, oxidative injury parameters and the expression of COX-2 gene in lung tissue were measured. The data indicated that sepsis induction reduced GSH, FRAP and increased LP, MPO, PGE2 and COX-2 gene but didn`t affected GST. Treatments of rats with DDW and E.Os alone and together as well as indomethacin have been effective in increasing the GSH, FRAP and decreasing the LP, MPO, PGE2 and COX-2. Histopathological examinations indicated that sepsis caused lung tissue damage and this damage decreased by DDW and E.O treatment. So sepsis causes oxidative lung tissue damage and the using of deuterium depleted water and Mentha longifolia can prevent injuries through modulation the oxidative stress/antioxidant parameters.