Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran

Author(s):

Ali Ehsan SHAHBAZI , Massoud SAIDIJAM , AmirHossein MAGHSOOD , Mohammad MATINI , Moosa MOTAVALI HAGHI , Mohammad FALLAH*

Message:
Article Type:
Short Communication/Paper (دارای رتبه معتبر)
Abstract:
Background

Hydatidosis is a cosmopolitan zoonotic infection and Hamadan Province in the west of Iran is one of the most important foci of human hydatidosis in Iran. The aim of the current study was the genetic characterization of hydatid cysts operated from humans in Hamadan Province.

Methods

Seventy-two hydatid cysts samples including 50 paraffinized and 22 fresh human hydatid cysts collected from different hospitals in Hamadan Province, western Iran. The cysts' DNA genome was extracted by kit and PCR was performed for amplifying the fragments of 400 and 450bp for nad1 and cox1 mitochondrial genes, respectively. Genotype diversity and sequence variations of the cysts' isolates were studied by related software.

Results

DNA from all (100%) paraffinized and fresh hydatid cysts samples extracted successfully. All paraffinized and fresh hydatid cysts samples were amplified by PCR assay using nad1gene, however, only 18 and 8 samples from paraffinized and fresh hydatid cyst samples was amplified using cox1 gene, respectively. The sequences analysis indicated that, 98.61% the Echinococcus granulosus samples were belong to the genotype G1 and 1.39% were G3 genotype.

Conclusion

Genotypes of E. granulosus in human samples in Hamadan Province are G1 and G3 and these findings are proved by phylogenic analysis.

Keywords:

Echinococcus granulosus , Genotypes , nad1 protein , cox1 protein , Iran

Language:
English
Published:
Iranian Journal of Parasitology, Volume:15 Issue: 2, Apr-Jun 2020
Pages:
259 to 265
https://magiran.com/p2136149  
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