Phylogenetic analysis of ND6 gene of mitochondria genome in native chickens of Sistan and Baluchestan

Today, genes in the mitochondrial genome are widely used as a strong genetic marker.The purpose of this study was to determine sequence of ND6 gene of the mitochondrial genome of Khazak chicken and investigate the phylogenetic relationship of this region of the genome with other chicken breeds and some fowls of around the world. 20 blood samples of wing vein were collected from Khazak chicken of Research Center of specific livestock of Sistan and Baluchestan province. DNA was extracted from whole blood. Then, the ND6 gene along with a portion of upstream and downstream tRNA (854 bp) was amplified from mitochondrial genome by specific primers in Polymerase Chain Reaction. Samples were sequenced after purification and genetic diversity was investigated within the population. After Download similar sequences of the other mitochondrial genome from the chicken breeds and some fowls of the NCBI, phylogenetic analysis was carried out. The results showed that there was no nucleotide difference between ND6 sequences of native Khazak chicken. The results of the phylogenetic tree from native Khazak chicken with other breeds showed that the highest genetic similarity was observed from this region of the mitochondrial genome of native Khazak chicken with HuangLang, Zhuxiang, Fiji, Philippines, Hengshan Yellow, Jinhu Wufeng and Red jungle fowl breeds which indicates that there are a high genetic relationship between the native Khazak chicken with the Japanese and Chinese (southeastern Asia) chicken breeds. The least genetic similarity of ND6 sequence from native Khazak chicken was observed with the Grus japonesis.