Quantitative evaluation of human sperm viability using MTT assay: A laboratory study

Author(s):

HamidReza Momeni* , MohammadHussein Abnosi , Najmeh Eskandari

Message:
Article Type:
Short Communication/Paper (دارای رتبه معتبر)
Abstract:
Background

3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability.

Objective

This study was performed to assess human sperm viability using MTT assay.

Materials and Methods

In this laboratory study, human-ejaculated semen samples (n = 56 from different donors) were used. The sperm viability was determined using quantitative MTT assay and the sperm motility was assessed according to World Health Organization guidelines. Sperm viability and the correlation between sperm viability and motility were analyzed.

Results

Data revealed a marked positive correlation between MTT reduction rate and the percentage of viable spermatozoa. The Pearsonchr('39')s correlation coefficients also showed a significant correlation between sperm viability and motility.

Conclusion

MTT assay which is based on mitochondrial functionality is a reliable method for evaluating human sperm viability and could be used as a diagnostic test for predicting sperm fertilization ability in clinical settings.

Keywords:

Human sperm , Viability , MTT assay

Language:
English
Published:
International Journal of Reproductive BioMedicine, Volume:18 Issue: 11, Nov 2020
Pages:
983 to 988
https://magiran.com/p2194993  
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