The Effect of Eight-Weeks of Aerobic Training with Nano-eugenol Supplementation on Pancreatic Tissue UPC3 Gene Expression and MDA Serum Levels in Diabetic Rats

The use of exercise and herbal supplements to treat diseases and metabolic disorders has become popular among the people. Regarding the health-enhancing effects of aerobic training and eugenol, the present study aimed to investigate the effect of eight weeks of aerobic training with nano-eugenol supplementation on the gene expression of non-placental protein 3 (UPC3) in pancreatic tissue and serum malondialdehyde (MDA) levels in diabetic rats.

In this experimental study, 20 diabetic rats were divided into four groups of five rats: 1) control, 2) aerobic training, 3) nano-eugenol, and 4) nano-eugenol + training. To investigate the effects of diabetes induction on research variables, five healthy rats were placed in a healthy control group. For eight weeks, groups 3 and 4 received 2 mmol of nano-eugenol by gavage daily, and groups 2 and 4 performed aerobic training at 30 m/s five days a week. Kolmogorov-Smirnov tests, one-way analysis of variance with Tukey post- hoc test were used to analyze the data (P≤0.05).

Induction of diabetes had a significant effect on increasing UPC3 gene expression in pancreatic tissue and MDA serum levels (P=0.001). However, aerobic training with nano-eugenol had a significant effect on the reduction of UPC3 gene expression in pancreatic tissue (P=0.001) and serum levels (P=0.01).

It seems that aerobic training along with the use of nanoeugenol has more favorable effects on improving UPC3 gene expression levels and MDA serum levels than the either one alone in diabetes.