Improvement of Haemorrhagic Septicaemia Vaccine by Removing of Anaphylactic Agents

The anaphylactic reactions in caule and buffaloes following haemorrhagic septicaemia vaccination in different parts of Iran were reported. The free endotoxin as an allergic substance is a component of liquid phase of the vaccine. A dense culture of bovine Pasteurella multocida type 8:2 was prepared in tri ptose phosphate broth supplemented by yeast extract. The bacterial cells were separated by centrifugation and alum gel precipitation methods. The safety trials of the vaccines conducted in mice, rabbits, guinea pigs, callie and buftàlos. The shock reaction did not occur either in laboratory or farm animais, which were tested in both controlled and field conditions.