THE EFFECT OF TWO MONTHS OF CHIA SUPPLEMENTATION ON INFLAMMATORY AND ANTI-INFLAMMATORY RESPONSES IN WISTAR DIABETIC MALE RATS

Increased inflammation is a consequence of diabetes. Accordingly, the use of herbal medicine as a solution has been suggested. The objective of this study was to evaluate the effect of two months of chia supplementation in conjunction with inflammatory and anti-inflammatory responses in male Wistar diabetic rats.

36 diabetic rats were randomly assigned to 3 equal groups (control group, diabetes group, diabetes + chia group) and were subjected to 8 weeks of supplementation. Interleukin-1 and interleukin-13 were measured in soleus muscle tissue to examine the inflammatory and anti-inflammatory indicators. ANOVA and Tukeychr('39')s post hoc test were used for data analysis.

Although chia supplementation had aggravating effects on IL-1ß compared with the diabetic group, this effect was not statistically significant (p = 0.99). Also, IL-13 protein expression increased significantly in the diabetic control group by about 51% (p = 0.001), and in the diabetic group with chia supplementation by about 50% (p= 0.001) compared to the healthy control group. There was no significant difference between the two groups.

It seems that two months of chia supplementation with this dose and period of consumption could not have a positive effect on anti-inflammatory factors in male diabetic rats. Confirmation of this finding requires further research in this area.