Identification compounds fruit of Ziziphus jujube Mill hydroethanoly extract and evaluation of Its antifungal effect on blanched corn grains

Nowadays, studies have been developing in order to use antimicrobial properties of plants instead of using synthetic preservatives. Jujube is one of the famous herbs. Jujube (Ziziphus jujube. Mill) belongs to family of Ramnaseh and native to the central areas of the Iran plateau. The fruit, leaves and root of this plant have medicinal and wellness properties. In this study, evaluated some physico-chemical of jujube extract (Extraction yield, Moisture, water activity, Fat content, Ash, Brix) and its effect on the mold of Aspergillus flavus. Extraction of dried and Ground jujube fruit was done by soaking with Hydroethanol solvent. Chemical composition of the extract was determined by GC-MS method. So the evaluated minimum inhibitory concentration (MIC) and the minimum fungal concentration (MFC) of extract against Aspergillus fulvous (PTCC 5004) by the dilution method in a liquid medium. In the next step, different concentrations of the extract were sprayed on corn grains and after placing a disk containing suspension of Mold spores in each petri dish. Growth of mold on corn grains was measured during 7 days. The results showed that the hydroethanolic extract of jujube fruit has an anti-mold effect against Aspergillus fulvous and the minimum inhibitory concentration of extract in the culture medium was determined 350 μg / ml and there was not observed any lethality at concentrations less than 1000 μg / ml. The minimum inhibitory concentration of the extract in the blanched corn grain medium was 5000 μg / ml. Therefore, the hydroethanolic extract of jujube fruit has an inhibitory effect on Aspergillus flavus but does not have the inactivation effect on this mold and its spores.