Identification of Molecular Markers Associated with Oleic Acid Level in Spring Oilseed rape (Brassica napus L.)

Oilseed rape (Brassica napus L.) is one of the world ’ s main sources of vegetable oil. The quality of the oil derived from oilseed rape is determined by its fatty acid composition. Breeding oilseed rape for enhanced oleic acid is one of the most important breeding programs that increases oil shelf life. In order to identify molecular markers associated with high oleic acid, a doubled haploid population derived from a cross between the lines T099-5318-20 and DH12075, RAPD and ISSR markers and bulked segregant analysis method were used. Six markers were found to be associated with oleic acid. The markers UBC 2830 and UBC 5381650 accounted 43% and 34% of genetic variation for oleic acid, respectively. In order to make SCAR marker, UBC 2830 was cloned and sequenced. From sequenced information, specific primers were designed. SCAR marker produced a 399 bp fragment linked to oleic acid. The markers identified in this study and the SCAR marker should be useful tools for the selection of high oleic acid genotypes in oilseed rape breeding programs.