The Effect of Endurance Training on Gene Expression of Homeobox C8 (HOXC8) and Homeobox C9 (HOXC9) in Adipose Tissue of Male Wistar Rats

Homeobox C8 (Hoxc8) and Homeobox C9 (Hoxc9) are homeoproteins involving in white adipose tissue development. The aim of current study was to investigate the effect of endurance training on gene expression of Hoxc8 and Hoxc9 gene in subcutaneous white adipose tissue (WAT). For this, 16 wistar rats (5 weeks old; an average of 210 gram) were divided into two groups included: 1) Control (n=8) and 2) Endurance Training (n=8). The subjects of training group underwent continues endurance training (by average speed of 25 m/min; by average duration of 25 min) on treadmill for eight weeks (5 session pre week). To measure of gene expression of subcutaneous tissue were used the Real Time (RT) –PCR method. Data showed that gene expression of UCP1 was significantly higher in trained group than control (P=0.018). However, the gene expression of Hoxc8 and Hoxc9 were not significantly different between trained and control groups (Phoxc8=0.36; Phoxc9=0.52). The results of this study showed that the endurance training did not change the gene expression of Hoxc8 and Hoxc9 in subcutaneous WAT, probably suggesting that the endurance training could not change the feature of white adipose tissue with respect to whitening and browning of WAT.