The effects of toxin binders and prebiotics on performance, intestine microbial population, stress and antioxidant indexes of broiler chicks fed with diets contaminated with aflatoxin B1

This study was conducted to investigate the effects of toxin binders and prebiotics on growth performance, intestinal microbial population, stress andantioxidant indexes of broiler chicks fed diets contaminated with aflatoxin B1. In this study, 600 1-d-old mixed broiler chicks (Ross 308) wereinvestigated in 10 treatments with 6 replications and 10 chicks per replication. Experimental treatments included: Negative controls un-supplementedand supplemented with ASRI1 and ASRI2 toxin binders and prebiotic and positive groups un-supplemented and supplemented with ASRI1 andASRI2 toxin binders and prebiotic, ASRI1 +prebiotic and ASRI2 +prebiotic. Growth performance, stress indexes of heterophile, lymphocyte,bacterial population and stress indexes of super oxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) wereinvestigated. Dietary inclusion of aflatoxin into diet increased heterophile, heterophile:lymphocyte ratio and decreased lymphocyte (P<0.05), but toxinbinders and prebiotic alleviated effects of aflatoxin on heterophile and lymphpocyte. Thepopulation of E. coli and lactobacilli were significantlyhigher and lower in positive control compared to negative control (P < 0.05). The results also showed that the serum concentrations of antioxidantenzymes were significantly lower in negative control compared to positive control (P < 0.05). In sum, aflatoxin showed negative effects on growthperformance, intestinal microbial populations, stress and antioxidant indexes but toxin binders and prebiotic decreased its negative effects andinclusion of ASRI1(3kg/ton) was better than other toxin binders.