sayed abdoullah hosseini

Vitamins play an important role in improving the performance, immune system, and growth of birds. In recent years, due to the increase of the price of synthetic vitamin supplements and also due to the limitation of the use of commercial antioxidants, plant-based antioxidant sources have been considered in the poultry industry. Therefore, this study aimed to investigate the effects of herbal vitamin E supplements on production traits, humoral immune responses, and some blood parameters of broiler chickens.

In a completely randomized design 480 Arian broiler chickens (one-day-old, mixed sexes) were allocated to four treatments, four replicates, and 30 birds in each replicate. Experimental treatments included: 1. basal diet (Contains 25 IU of synthetic vitamin E), 2. basal diet + 25 IU synthetic vitamin E, 3. basal diet + 50 mg herbal vitamin E supplement, and 4. basal diet + 100 mg herbal vitamin E Supplement. In this experiment, weight gain, feed intake, and feed conversion ratio (FCR) were measured. On day 40, two birds per replicate were randomly selected, weighed, and killed, and then the entire empty carcass and parts of the carcass including breast, thigh, neck back, and wings, abdominal fat, and some organs including heart and spleen were weighed. One day 40 days, to measure the concentration of total protein, albumin, triglyceride, cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL), and the activity of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase enzymes, blood was collected from each replicate of 4 birds. On day 33 to measure antibody titer against Newcastle disease and influenza, 1 ml SRBC was injected into 4 birds in each repetition.

  The results showed that the experimental treatments had no effects on feed intake, body weight, and FCR. Also, carcass characteristics including carcass percentage, breast, thigh, back, and neck percentage, abdominal fat, heart, spleen, and blood parameters were not affected by experimental treatments. Antibody titer against sheep red blood cell injection had no significant effect.

In total, according to the recommendation of commercial strains, the requirement of vitamin E is 50 IU, therefore in case of providing 25 IU of synthetic vitamin E in the diet, it is possible to provide the rest of the need up to 50 IU by using the herbal vitamin E supplement (at the rate of 50 mg per kilogram of feed) considering there are economic considerations.

For this expriment 1440 Ross 308 male broilers (from 1 to 42 days of age) were used in a randomized complete block design with eight treatments in six blocks. Experimental diets included: soybean meal (control treatment), raw soybeans, full-fat soybeans extruded (15%) at 90 and 150°C, and each of them with 0.02% or without (0.0) protease enzyme supplementation. The use of raw soybean compared to soybean meal or extruded full-fat soybean significantly reduced feed intake, body weight and relative carcass weight, as well as increased feed conversion ratio and pancreatic weight (P<0.01). weight gain was improved in diets supplemented by protease enzyme compared to diets containing raw soybean (P<0.01). Feed conversion ratio was improved in diets containing raw soybean and soy bean meal by dietary protease supplementation (P<0.01). Extrusion temperature did not affect the growth performance of experimental birds. Diet containing raw soybean without enzyme increased blood cholesterol levels (P <0.05). According to the findings of this study, the use of protease enzyme in diets containing soybean meal or raw soybean can improve the performance of broilers However, such effect is less pronounced in diets containing extruded full-fat soybean meal.

This study was conducted to evaluate the effects of adding different levels of a domestically produced synbiotic to drinking water on production traits, carcass characteristics and the small intestinal microbial population of broiler chickens. Four hundred one-day-old Ross 308 broilers (a mixture of two sexes with equal proportion) were used in a completely randomized design with 4 treatments, 5 replicates, and of 20 birds per each replicate. Experimental treatments included adding levels: zero (control), 50, 100 and 150 g of synbiotic (Biopoul) per 1000 liters of drinking water. At 42 days of age, the addition of 50 or 100 g of synbiotic per 1000 liters of drinking water significantly decreased feed conversion ratio (P < 0.05). The increase of production index in the group receiving 100 g of Biopoul compared to the control showed a tendency (P = 0.08). Biopoul in the levels of 50, 100 and 150 g increased the total number of lactobacilli and decreased the number of yeasts and molds, coliforms and total gram-negative bacteria in the ileum compared to the control (P<0.05). According to the obtained results, the use of 100 g of synbiotic Biopoul per 1000 liters of drinking water of broiler chickens can improve the production index while helping to balance the small intestinal microbial population.

In this research the effect of Bioplus B2 probiotic and galactooligosaccharide prebiotic (GOS) on performance, egg quality, and some bloodparameters of commercial laying hens were investigated in a sample of 216 hy-line laying hens (w36) with the same average weight aging 50to 62 weeks for a period of 12 weeks in a completely randomized design with 6 treatments, 6 replications and 6 hens per each replication inthe cage system. Experimental treatments included 1) without additives (as control), 2) diets containing 0.1% Bioplus B2 probiotic, 3) to 6)diets; containing, 0.05, 0.1, 0.15 and 0.20 % of galactooligosaccharides prebiotic, respectively. The results indicated that both egg weightand mass was increased significantly (P<0.05) in the Bioplus B2 probiotic treatment. Egg production percentage, feed intake and feedconversion ratio were not significantly influenced by treatments. Using galactooligosaccharide at the levels of 0.05, 0.1 and 0.2 percentimproved dry eggshell weight significantly while the levels of 0.05, 0.15 and 0.2 percent increased eggshell weight per unit area compared tothe control (P<0.05). Total protein and albumin of serum increased in the Bioplus B2 treatment (P<0.05). Using Bioplus B2 probiotic and0.15% galactooligosaccharide significantly decreased blood cholesterol level (P<0.05). In general, the results showed that the using ofprebiotic galactooligosaccharide at the level of 0.15 % increases the weight of eggshell per unit area, decreases the blood serum cholesterol,and improves some performance traits compared to control group, therefore it can be used instead of probiotic Bioplus B2.

This study was conducted to investigate the effects of toxin binders and prebiotics on growth performance, intestinal microbial population, stress andantioxidant indexes of broiler chicks fed diets contaminated with aflatoxin B1. In this study, 600 1-d-old mixed broiler chicks (Ross 308) wereinvestigated in 10 treatments with 6 replications and 10 chicks per replication. Experimental treatments included: Negative controls un-supplementedand supplemented with ASRI1 and ASRI2 toxin binders and prebiotic and positive groups un-supplemented and supplemented with ASRI1 andASRI2 toxin binders and prebiotic, ASRI1 +prebiotic and ASRI2 +prebiotic. Growth performance, stress indexes of heterophile, lymphocyte,bacterial population and stress indexes of super oxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) wereinvestigated. Dietary inclusion of aflatoxin into diet increased heterophile, heterophile:lymphocyte ratio and decreased lymphocyte (P<0.05), but toxinbinders and prebiotic alleviated effects of aflatoxin on heterophile and lymphpocyte. Thepopulation of E. coli and lactobacilli were significantlyhigher and lower in positive control compared to negative control (P < 0.05). The results also showed that the serum concentrations of antioxidantenzymes were significantly lower in negative control compared to positive control (P < 0.05). In sum, aflatoxin showed negative effects on growthperformance, intestinal microbial populations, stress and antioxidant indexes but toxin binders and prebiotic decreased its negative effects andinclusion of ASRI1(3kg/ton) was better than other toxin binders.

The limitation of feed sources in Iran and all over the world has increased attentions to use alternative feedstuffs. Insect production is one the recommended approaches to improve food security for human and animals. Insects are rapidly grown and proliferated and because of being cold-blooded have appropriate feed conversion ratio. They also can convert agricultural residues into biomass contained proper value of energy and protein. This article has been focused on introduction of some insects used in poultry neutrino (including cricket, mealworm, black soldier fly larva, house fly larva, grasshopper) and earthworm, followed by reviewing the poultry nutritionist’s results about them, and ended up with pointing the challenges regarding to the extension of insect usage in poultry nutrition. The results of the researches indicated the appropriate levels of crude protein (42.1-63.3%) and metabolizable energy (3833-3298 kcal/kg) in evaluated insects or larva and the possibility of using them in poultry feed (commonly 5% of diet). Regarding the challenges of insect production and usage, some points which need especial attention are included: developing legal instructions for authorizing insect production and usage, choosing safe and proper insect species, type of litter and substrate of feeding, the chitin content of exoskeleton, as well as appropriate marketing and cultural education of the poultry farmers and human consumers of the poultry products.

This study was conducted to investigate the effects of anti-stress feed additive containing probiotic Protexin®, vitamin C and betaine in three commercial broiler strains on immune responses, some blood parameters and oxidative stability of breast muscle under heat stress conditions. A total of 720 one-day-old broiler chicks were used in a completely randomized design as a 2×3 factorial arrangement consisting 2 levels of additive (with and without) and three broiler strains (Arbor Acres, Ross 308 and Cobb 500) with 6 treatments, 4 replicates and 30 birds in each replicate. The birds were exposed to heat stress (37 °C) for 8 hours daily from 22 to 42 days of age. In addition of performance parameters, humoral immune response was measured by intravenous administration of 5% sheep red blood cell (SRBC) suspension and primary and secondary antibody titers were determined. At 42 day of age, blood samples were collected to measure blood serum parameters including total protein, cholesterol, triglyceride, HDL and LDL. The heterophile to lymphocyte ratio and also glutathione peroxidase and super oxide dismutase enzyme activities were evaluated at 42 day of age. Among the evaluated parameters, only LDL concentration was affected by experimental strains (p < 0.05) and the minimum serum LDL concentration was observed in Arbor Acres strain. Generally, the strain and feed additive used in this experiment had no significant effect on immune response and breast muscle oxidative stability of heat-stressed broilers.

This experiment carried out to determine the nutritional value of 18 sorghum forage varieties and to introduce superior cultivars using multicriteria decision-making technology. They sow in early June and harvested in mid-November. Random samples were taken from the center of each experimental plot in a way that ten plants were cut from 10 cm above soil. These samples were cut into 3 to 5 cm pieces, eachcultivar had three samples, in total 54 samples were collected. Yield and nutrient contents such as, crude protein, crude ash, ADF, NDF, water-soluble carbohydrates, starch and lignin measured in the experimental cultivars. The metabolizable energy of sorghum cultivars estimated by gas test method using the amount of gas produced in 24 hours and the amount of crude protein contained in it. Sorghumcultivars ranked based on 11 integrated traits. Among the domestic cultivars, Spidfeed ranked fifth among all varieties, followed by KFS-18 ranked seven and pegah ranked 10th and KFS-2 ranked 12th with moderate quantitative and qualitative performance. Overall, based on the results of the multi-criteria decision-making method, it was found that the top three sorghum cultivars are Siloking, FGCSI12 and PHFS-27, respectively, which are recommended for sorghum forage production and silage for dairy farmers.

This study was aimed to investigate the effect of adding dried dairy wastes to the starter diet on growth performance in Holstein dairy calves. A total of 28 female newborn Holstein dairy calves were randomly allocated to four groups (n=7/group) and after two weeks of habituation to experimental conditions and diet, in a completely randomized design, individually fed starter diets containing 0 (control), 10, 20, or 30% dried dairy waste up to eight weeks of age. Feed intake, weight gain, and feed efficiency (gained weight/consumed feed) were measured weekly, during the experimental period. Results showed that body weight, weight gain, feed intake, and feed efficiency were not affected by the treatments. Interactive effect of treatments by week showed that the highest weight gain was for calves fed by the diet containing 10% dairy waste or control group at first week. However, the highest gain was recorded for those that received 30% dairy waste at week five of the experiment (P<0.05). In conclusion, the results of the present study revealed that the inclusion of dried dairy products up to 30% of calves’ starter diet had no adverse effect on performance, and therefore can be used as an affordable and valuable nutritional by-product of the dairy industry in the nutrition of calves.

An experiment was undertaken to determine the optimum level of corn germ (high-oil content) in broilers diet. This experiment was conducted in a completely randomized design with 5 experimental groups, 5 replicates and 30 birds per each experimental unit from 15 to 42 days of age. Experimental groups were included birds received levels of zero (control), 5, 10, 15 and 20 percentage of corn germ in diet. During the experiment performance traits, productive index, feed cost per kilogram of live body weight, humoral immune responses and intestinal histomorphology were evaluated. To determine the optimum level of corn germ in diet, technique for order preference by similarity to ideal solution (TOPSIS) and maximizing economic profit method were applied. According to the results, optimum level of dietary corn germ inclusion based on TOPSIS and economic methods were 6.59 and 6.98 percent, respectively.

In order to determine effect of various sources of manganese, zinc and copper (Sulfate, foreign and Internal organic) and requirement levels (100, 50% requirement) on performance and immune response of broilers, an expriment was conducted using 480 commercial CAB 500 hybrid broilers in a 2×3 factorial arrangement with completely randomized design. During the experiment, live body weight (LBW), feed intake (FI) and feed conversion ratio (FCR) were measured. At the end of the experiment, blood sampling was done and total antibody titer against sheep's red blood cell (SRBC), antibody titer against Newcastle Disease virus (ND) and differential count of white blood cells (WBC) were measured. The results showed that mineral sources did not affect the performance of broilers. The sources of minerals had a significant effect on livability percentage (P<0.05) and the highest livability was observed for organic (foreign and local) treatments. Usage of local organic manganese, zinc and copper caused a better response to sheep's red blood cell (SRBC) (P<0.05) relative to foreign form. Moreover, there was significant difference among the treatments in terms of weight (P<0.05), feed conversion (P<0.05) and production index (P<0.05) and treatment with 100% requirement level had maximum impact. The birds fed with 50 % requirement showed a better response to SRBC (P<0.05). According to the results of this experiment, the organic forms of the three manganese, zinc and copper produced in Iran can simultaneously, meet the needs of broilers and can be replaced with inorganic and the foreign sources. In addition, the 100 % requirement level of manganese, zinc and copper, regardless of source, is recommended to achieve the maximum growth performance and 50 % requirement level is recommended for better immune function.

The effects of two sources of organic zinc on performance and immune responses of broiler chickens were evaluated by using seven-hundred and fifty day-old Arian broiler chickens which randomly allocated in 30 experimental units in a completely randomized design as a 2×3 factorial with two sources of organic zinc (zinc- methionine and zinc- glycine) and three levels of dietary zinc (40, 80 and 120 mg/kg). During the experiment, live body weight (LBW), feed intake (FI) and feed conversion ratio (FCR) were measured weekly. At the end of the experiment (age 42d) two birds from each replicate were bleed and total antibody titer against Sheep Red Blood Cell (SRBC), antibody titer against Newcastle Disease virus (ND) and differential count of white blood cells (WBC) were measured. Dietary inclusion of zinc-methionine improved LBW (day 42), FI (days 0-28) and FCR (days 0-14) in comparison with zinc- glycine (P

The aim of this experiment was to assess the effects of encapsulated oregano oil on microbial population, morphology, length and weight of different parts of digestive tract of Aryan broiler chicks. A total of 625 Arian broiler chicks were used in a completely randomized trail with 5 treatments and 5 replicates at the age of 1 to 42 days. Treatments were including: 1- basal diet (negative control), 2- basal dietꚺᮊ譢 (0.1 g Protexin/kg), 3- basal diet橻扮⨭ (0.15g Avilamycin/kg), 4- basal dietꊷꁪ oil (0.2 g/kg) and 5- basal diet穷᪦쮥櫗 oregano oil (1 g/kg). The results showed that broiler performance at the end of 42 days were not affected by treatments (p0.05). Finally, according to result of intestinal microbial count, it seems that encapsulated oregano essential oil can improve the intestine microbial population and bird's health.

In order to determination of the share of cost factors affecting the cost of chick live weight per kilogram by using multi-criteria decision-making، a study were done in Tehran province. Data were collected through questionnaires. Farms were divided to three cost ranges، 1- less than 35،000، 2-between 35،000 to 40،000 and 3-over 40000 Rails based on production cost per kg of live weight. Performance parameters، including body weight and feed conversion ratio were not different among three groups (p >0. 05). To determine the share of cost factors، the difference between the groups were analyzed by using multi-criteria decision-making methods. Water and additives، staff، vaccines and fuel are the most factors that influence the difference among 3 groups، so they made a big difference in final live weight price. But the effects of diet cost and insurance had lowest effects on final price. The total cost of feed، chicks، personnel، fuel، rent، vaccinated and litter، respectively، 66. 75، 12. 4، 5. 03، 2. 8، 2. 6، and 1. 38 percent of the production costs themselves. The results showed that the average production cost 38،000 Rrails in Tehran province in the summer of 2014.

From a total of 656 broiler farms with the permission of exploitation in Guilan province, data was gathered from 20% of active farms including 85 units (Capacity of over 1663000 portions of broiler chickens) to evaluate the management of nutrition and nourish using questionnaire. The questionnaires were completed by the interview method. The methods of making feed, feed formulation, the shape of grain, the frequency of feeding, the type of dietary supplement and food additives, the use of experts, feed conversion ratio, causes of mortality, the age of mortality, drinking and feeding systems, and how to use fine nourishing (in the shape of supplement or concentrate) were considered in this study. The performance was calculated for each broiler farm. According to the production index, the farms were divided into three groups of weak (200+25), medium (250+25), and good (300+25) and their differences were compared. According to the results, among the managerial factors, the factors such as: feeding system, water quality, the conformity of ration in nutrition with the needs of commercial strains and drinking management, have the most portion in creating the three groups of good, medium and weak, among the broiler rearing farms. Thus, in the study of each group’s feeding system, the good group has the highest percentage of using automatic systems (86/60) and the weak group has the highest percentage of using manual systems. In comparing three groups regarding the drinking water quality, it was observed that good, medium and weak groups drink 92%, 61.84%, and 75% fresh water, respectively. Furthermore, in the weak group, most of the farms have used the water with tolerable hardness or saltiness. Finally, the conformity of strain’s requirement with diet was examined among groups. The results showed that 16.5% of units followed this conformity and the percentages of the good, medium, weak groups was 11.12%, 28%, 53.5%, respectively.