Investigation of phylogenetic relationships and genetic diversity of Seidlitzia rosmarinus populations by ISSR marker in some regions of Iran

The genus Seidlitzia has four herbaceous species viz. S. florida (M.B.) Bge. ex Boiss., S. cinerea (Moq.) Bge. ex Botsch. and S. stokii that are herbaceous, and S. rosmarinus (Ehrenb.) Bge. ex Boiss. is shrubby belong to the family Amaranthaceae. In the present study, ISSR molecular markers were used to evaluate the genetic diversity among 85 plant samples from 17 populations of S. rosmarinus. Four random primers were used to perform ISSR reactions and in total 221 DNA bands were obtained. After forming 0 and 1 matrix with the help of GenAlex and NTSYS software, it was used to interpret ISSR analysis data. Molecular analysis of variance (AMOVA) was performed to estimate genetic diversity out of 85 individuals. Of total diversity, the highest amount of genetic diversity (68%) was related to intra-population diversity, 28% of inter-population diversity, and 4% of inter areas was calculated. MCMC analysis using Structure 2.3.4 software was used to investigate the genetic diversity populations of S. rosmarinus. Main coordinates analysis by the PCOA method was also used for extract maximum information from molecular data.