Effect of Germinated Black Rice Krisna Extract on Fasting Blood Glucose and Body Weight in Diabetes Mellitus Rats

Diabetes mellitus (DM) is a non-communicable disease that is generally associated with fasting blood glucose (FBG) level of >126 mg/dL and weight loss. Germinated black rice krisna (GBRK) extract contains phytochemicals with antioxidant and anti-diabetic properties. The purpose of this study was to assess the impact of GBRK extract on FBG level and body weight (BW) in rat model of DM. In an in vivo pre- and post-test study 36 male Wistar rats with BW design, of 150-200 g were enrolled and divided into 6 groups, including control (C), negative control (NC), GBRK 535 mg/kg BW (P1), GBRK 1070 mg/kg. BW (P2), and GBRK 2140 mg/kg BW (P3). Diabetes was induced using streptozotocin (STZ) and nicotinamide (NA) and the intervention period was 14 days. Measurement of FBG level was by quantitative enzymatic colorimetric test of Glucose Oxidase Phenol 4-Aminoantipyrine (GOD-PAP) and body weight was determined by a digital scale. The administration of GBRK extract in P1, P2, and P3 groups could significantly reduce the FBG (p <0.01). GBRK in P1, P2, and P3 groups could also significantly improve the body weight to normal state too (p <0.01). GBRK extract at doses of 535, 1070, and 2140 mg/kg BW for 14 days was demonstrated to improve metabolic disorders in diabetes by reducing FBG level and improving the BW to the normal state.