The effect of Asparagus officinalis extract on Arabi ram semen quality during storage in liquid condition at 4 ◦C

The study aimed to investigate the effect of asparagus extract on quality semen of Arabian rams at different storage times at a temperature of 4degrees Celsius. Sperm collection was performed from 10 Arab rams with an average age of 2.5 years per week (once a week) for six weeks. Afteradding 0, 1.5, 3 and 4.5% asparagus extract to the semen diluent, sperm quality parameters were evaluated at 0, 24, 48, and 72 hours after storage inliquid condition. At zero hour, sperm viability in diluent had 3% asparagus extract was higher than diluent containing 0 and 4.5% extract (P<0.05).After 24 hours of semen storage, the parameters of total motility, progressive motility, plasma membrane integrity and viability were higher in spermdiluent containing 1.5 and 3% of the extract (P<0.05). Qualitative parameters of sperm in 48 hours after sperm collection and storage in diluentcontain 3% of extract had significant and better performance (P<0.05). The highest rate of plasma membrane integrity, progressive motility and totalsperm motility was observed in diluent containing 3% extract in 72 hours after sperm storage (P<0.05). According to the results of this study, the useof asparagus extract in the diluent at the rate of 3% improves the sperm quality of Arabian rams after cooling conditions.