جستجوی مقالات مرتبط با کلیدواژه "motility" در نشریات گروه "علوم دام"

Studies had shown that excessive production of free radicals during sperm processing decreases its quality parameters including motility, viability, and fertilizing ability. Therefore, it seems necessary to use a compound with antioxidant properties during this process. Evening primrose oil has antioxidant properties due to its phenolic compounds. the purpose of this research is to investigate the effect of adding evening primrose seed oil to freezing diluent on the quality of ram sperm after freezing and thawing process.

In this research, the semen of 5 adult Romanov rams with an average age of 3 to 4 years and average weight of 60 to 65 kg were used. Semen collected using artificial vagina from trained rams twice a week. The treatments included control (without evening primrose seed oil), treatment 1 with 25 microliters EPSO, treatment 2 with 50 microliters EPSO, treatment 3 with 100 microliters EPSO. The collected semen sample to laboratory and examined in terms of volume, concentration and motility. Semen samples were diluted with a diluent based on Tris- lecithin soy and frozen. After thawing, parameters of general mobility and progressive mobility and other parameters related to mobility were evaluated. The survival percentage, membrane integrity, mitochondrial activity and peroxidation were determined.

The results showed that the level of 25 microliters of evening primrose seed oil improve the motility of the whole sperm compared to the control group, and no significant difference was observed in other experimental treatments compared to the control group. There was no significant difference between the 25 and 50 microliters treatments with the control group in the progressive parameter, but there was a significant difference with the 100 microliters treatment compared to the other treatments and the control treatment(P<0.01).The results of lipid peroxidation showed that the lowest amount of Malon-di-aldehyde production was related to the 25 microliters treatment but no significant difference was observed with the control treatment. The results of the apoptosis test showed that the addition of levels of 25 and 50 microliters of evening primrose seed oil decreased the amount of apoptosis compared to the control group and the level of 100 microliters of evening primrose seed oil(P<0.01). Also, survival in the treatment of 25 and 50 microliters compared to others The treatments had a significant difference and increased survival(P<0.01). And finally, dead sperms in the 25 microliters treatment were less compared to other experimental groups, and this caused a significant difference in this trait compared to other levels(P<0.01).

In general, the addition of evening primrose seed oil in the freezing diluent leads to an increase in the viability and health of the plasma membrane of sperms after thawing. Adding 25 microliters of evening primrose seed oil to the diluent caused a significant difference in the parameters of lipid peroxidation and apoptosis compared to the control treatment.

The study aimed to investigate the effect of asparagus extract on quality semen of Arabian rams at different storage times at a temperature of 4degrees Celsius. Sperm collection was performed from 10 Arab rams with an average age of 2.5 years per week (once a week) for six weeks. Afteradding 0, 1.5, 3 and 4.5% asparagus extract to the semen diluent, sperm quality parameters were evaluated at 0, 24, 48, and 72 hours after storage inliquid condition. At zero hour, sperm viability in diluent had 3% asparagus extract was higher than diluent containing 0 and 4.5% extract (P<0.05).After 24 hours of semen storage, the parameters of total motility, progressive motility, plasma membrane integrity and viability were higher in spermdiluent containing 1.5 and 3% of the extract (P<0.05). Qualitative parameters of sperm in 48 hours after sperm collection and storage in diluentcontain 3% of extract had significant and better performance (P<0.05). The highest rate of plasma membrane integrity, progressive motility and totalsperm motility was observed in diluent containing 3% extract in 72 hours after sperm storage (P<0.05). According to the results of this study, the useof asparagus extract in the diluent at the rate of 3% improves the sperm quality of Arabian rams after cooling conditions.

The use of the plant Tribulus Terrestris can improve semen quality in some mammals and birds. However, little information is available about adult male lambs. The purpose of this experiment was to investigate the effect of different levels of Tribulus terrestris on quantitative and qualitative characteristics of sperm of mature male lambs. In this study, 18 lambs of the arabi lambes age 3 monthes and average body weight 16.14±2 were divided into three groups of six in a completely randomized design, and received different levels of Tribulus terrestris including three groups: 1) without Tribulus terrestris (control), 2) 15 g/kg DM Tribulus terrestris and 3) 30 g/kg DM Tribulus terrestris for 5 months. The spermatogenesis was carried out by the electrocautery device two times a week from lambs after feeding Tribulus Terrestris. Quantitative and qualitative semen parameters including seminal volume and pH, sperm concentration, motility, survival and morphological abnormalities of sperms were evaluated. Results showed, the lowest semen volume was in the control group (p < 0.05). The highest concentration, motility and survival of sperm were from 15 g of Tribulus terrestris (p < 0.05). The level of 30 g of Tribulus terrestris showed the highest morphological abnormalities of sperm (p < 0.05). Mean pH was not affected by experimental treatments (p < 0.05). In conclusion, considering to all the measured parameters, adding 15 g of Tribulus terrestris per kilogram dry matter to the diet improved the quantitative and qualitative characteristics of sperm in the freshly mature arabi lambs.

This study was conducted to determine the suitable dose of gamma rays for increasing sperm motility and viability after thawing and to assess its effects on malondialdehyde concentration and the rate of sperm DNA strand breaks. Sperm samples in liquid nitrogen were irradiated at doses of zero, 0.1, 0.3, 0.5, 0.7 and 0.9 Gy. The sperm quality and quantity parameters were evaluated before and after irradiation using CASA system. Sperm morphology and viability were evaluated using Eosin-Nigrosin staining. Malondialdehyde concentration was determined based on thiobarbituric acid method and DNA strand breaks analyzed by Comet assay. Data were analyzed based on randomized complete block design by SAS Software. The results showed that irradiation influenced (P<0.05) on motility and viability of sperms. The semen malondialdehyde concentration had no significant differences among treatments (P>0.05). Based on the Comet assay result, DNA strand breaks parameters had no significant differences with the control group, except at dose of 0.9 Gy. The results of this study demonstrated that gamma irradiation at dose of 0.7 Gy could enhance the motility and viability of sperms after thawing without negative effect on semen and sperm quality.

This study aimed to evaluate the effect of L-arginine on qualitative characteristics and fatty acid profile of Ross 308 aged broiler breeder roosters’ sperm. Twelve breeder roosters at age of 52 week were used in a completely randomized design with three treatments and four replicates in each treatment for eight consecutive weeks. Experimental groups were consisting diets with levels of 0.52 (Ross recommendation 308), 0.68 and 0.83 present of arginine amino acids. Semen collection was performed every 14 days and in weeks 54, 56, 58 and 60 were tested. At the end of the trial, fatty acid profile of sperm was also evaluated. On week 56, semen volume in roosters with 0.68 present arginine was higher than other treatments (P<0.05). On week 60, levels of 0.52 and 0.68 percent arginine in semen volume, percentage of  total and progressive motility were higher than the level of 0.83 percent of arginine (P<0.05). On week 58, the percentage of abnormal sperm were lower in 0.68 and 0.83 percent arginine treatment compared to 0.52 percent arginine (P<0.05). Semen concentration, sperm plasma membrane functionality and sperm fatty acid profile were not affected by treatments used in this study. It can be concluded that 0.68 percent of arginine (30% higher than recommendation) of diet improve some qualitative sperm parameters in aged broiler breeder roosters.

Semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. Cryopreservation of spermatozoa is becoming more important because of new clinical requirements and current clinical practice. Although frozen-thawed semen has great practical benefits for reproduction, it is widely reported that the cryopreservation process involving cooling, freezing, and thawing induces serious detrimental changes in sperm functions. The viability, motility and membrane integrity of mammalian spermatozoa decrease during the cryopreservation process. Many studies exist as regards the effects of antioxidants on the cryopreservation aimed at improving the quality of post-thaw semen. Antioxidant molecules could decrease the impact of oxidative stress and therefore improve Sperm quality following the freeze–thawing process. Melatonin (N-acetyl-5-methoxytryptamine), a derivative of tryptophan, is mainly synthesized and secreted by the pineal gland during the night in reaction to changes in light levels. Melatonin can stimulate the activity of antioxidant enzymes such as SOD and GSH-Px. melatonin scavenges a variety of reactive oxygen and nitrogen species in vivo and in vitro, signifying it has a powerful non-enzymatic antioxidant property. It has been shown that the spermatozoa undergo a freeze–thawing process produced high concentrations of reactive oxygen species. The aim of this study was to investigate the effects different levels of melatonin supplementation (0, 0.5, 1, 2 and 4 mM/ml) in extender on semen characteristics the Arabic ram after freezing-thawing. This research was performed at Ramin Agriculture and Natural Resources University of Khuzestan in the fall in 2015. Semen samples were collected from 6 Arabic ram with an average weight 73 ± 3 kg by electro ejaculator twice a week. Sperm samples motility were assessed by Computer Aided
Sperm Analysis (CASA) after freezing and thawing. The pH, viability, abnormal sperm percentage, membrane integrity and total antioxidant capacity were also assessed after freezing-thawing process. Pearson correlation test was used to assess correlation of melatonin with routine sperm parameters (pH, viability, abnormal sperm percentage, membrane integrity and total antioxidant apacity of plasma). Data analysis was performed using SAS software. P<0.05 was considered significant. Results and Discussion the Results of this experiment showed that the diluent of ram semen containing 0.5 mM/ml of melatonin improved the motility, membrane integrity and viability of Arabic ram spermatozoa compared to the control. The total antioxidant capacity was highest in diluent contained whit 1 mM/ml of melatonin. The effect of melatonin on the pH of semen at all levels was not significant. Cryopreservation causes an irreversible damage to enzymatic activity and sperm organelles leading to a reduction in the sperm kinetic parameters. Composition of extender may also affect the freeze ability of spermatozoa and their fertilizing ability. Many studies reported that melatonin has beneficial effects on preservation of mammalian sperm function and improves the microscopic parameters of spermatozoa. Melatonin supplementation to ram semen freezing extender protected spermatozoa from the cryopreservation injuries, as proved by post-thaw viability, motility, intracellular ATP concentrations, DNA integrity, and fertilizing ability. It is suggested that melatonin stimulates the activities of antioxidant enzymes. In consequence, melatonin reduces the number of free radicals, ROS, and also may increases the production of molecules protecting sperm cells against oxidative stress. As a conclusion, supplementation of melatonin in the freezing medium can counteract the adverse effects of the freeze–thawing process on the motility, viability, normal morphology and plasma membrane integrity in ram spermatozoa. The results were suggested that the protective effects of melatonin on spermatozoa were associated with a reduction in LPO as a consequence of increasing the TAC and antioxidant enzymes activity. Cryopreservation of sperm is an applicable technique in infertility management but it may influence the post-thaw qualities of sperm, including morphology, motility, viability and DNA integrity. In this research, we show that supplementation of cryopreservation extenders with melatonin provide a cryoprotective effect on pH, viability, abnormal sperm percentage, membrane integrity and total antioxidant capacity. Overall addition of 0.5 mM/ml of melatonin to the extender improved the most of spermatozoa quality characteristics as well as total antioxidant capacity of semen after freezing-thawing process in Arabic ram. In the current study, we observed no correlation between melatonin and pH, viability, abnormal sperm percentage, membrane integrity and total antioxidant capacity of plasma.

Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates per each group. Semen samples were collected on days 0, 14, 28, 42 and 56 of experiment. Results showed that 100mg rosemary significantly improved several traits of sperm such as Amplitude of Lateral Head Displacement (ALH), Average Path Velocity (VAP), and Straight Line Velocity (VSL) on days 42 and 56, and sperm Membrane Integrity (MI), sperm viability, Linearity (LIN) and Total Motility (TM) on day 56 (P

Cadmium, as an environmental pollutant, can exert oxidative stress on spermatogenesis and sperm morphology. This study was performed to investigate not only the effect of cadmium on viability, motility and acrosome integrity of ram sperm but also examine the protective role of silymarin, as an antioxidant, on cadmium toxicity. Epidydimal sperm obtained from Farahaniˊs ram divided into four groups: 1. sperm at 0 hour 2. Sperm incubated for180 minutes (control) 3. sperm treated with cadmium chloride for 180 minutes and 4. sperm treatment with silymarin+cadmiumchloride for 180 minutes. MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method was used to assess sperm viability. Sperm motility was done according to World Health Organization guidelines while acrosome integrity in the sperm was evaluated by comassie brilliant blue staining. Data were analyzed using one way ANOVA followed with Tukeyˊs test. The percentage of sperm viability, motility and acrosome integrity was significantly decreased in cadmium chloride-treated group compared to the control. In silymarin+cadmiumchloride group, silymarin could compensate the adverse effect of cadmium chloride on sperm parameters (except sperm motility) compared to cadmium chloride group. Silymarin as a potent antioxidant prevented adverse effect of cadmium on some ram sperm parameters.