The effect of carbon nanoparticles on physiological characteristics of Red fleshed apples and Malling Merton 106

Micropropagation plays an important role in the production of vigorous and healthy plants in apple tree. Carbon nanoparticles have a significant effect on increasing the growth and germination of different plant species under in vitro condition. In this study, the effect of different kinds of nanoparticles on explant proliferation of two apple genotypes (red fleshed and Malling Merton 106) was investigated. The treatments were multi walled of carbon nanotubes and graphene oxide in 3 concentrations of 0, 50 and 100 mg L-1. In order to investigate the entrance and accumulation of carbon nanoparticles in the plant, a fluorescence microscope with a light spectrum of 530 nm was used. Carbon nanotubes and graphene oxide showed a positive effect on explant proliferation by increasing leaf production and stem length compared to the control. The both treatments improved leaf growth by decreasing plastochron. In this study, the penetration of nanoparticles into leaf tissue was observed. The effect of graphene oxide was most than carbon nanotubes.