Effect of Electromagnetic Fields with 0.25 and 2.5 ml Tesla strength, in two type of continuous and discontinues radiation on miR-21 and miR-29 Genes Expression in Gastric Cancer Cell Line

Bio electromagnetic studies, and the role of electromagnetic field radiation with very low frequency is the important environmental factors associated with the beginning and treatment of cancers. In this study, the variation of miR-21 and miR-29 expression in the gastric cancer cell line was investigated when was exposed under the influence of electromagnetic fields with 0.25 and 2.5 ml Tesla strength, in two type of continuous and discontinues radiation.

Gastric cancer cell line culture, was exposed to the electromagnetic radiation system including 0.25 and 2.5 ml Tesla field strength and in continuous and discontinues radiation, and was compared with a non-radiation control group. After RNA extraction from cells, using the cDNA synthesis and by Real Time PCR variations of two microRNAs expression was explored and results was statistical analyzed.

According the results, cell viability percent in electromagnetic field radiation compare to the control group, was showed significant decrease. Eexpression of miR-21 and miR-29 was showed significant increase due to increase the electromagnetic field radiation strength. As also, nearby over expression of miR-21 and miR-29 in two type radiation, cell viability percent was shown significant decline.

Finding demonstrated radiation with very low frequency electromagnetic field in the gastric cancer cell line has led to over expression of the miR-21 and miR-29, which confirmation beside future studies, it's could be proposed as molecular biomarker for gastric cancer.