Investigation of phylogenetic relationships and genetic diversity of population (Seidlitzia rosmarinus) by RAPD marker in some regions of Iran

The genus Sedlitzia (Amaranthaceae) includes four herbaceous and one shrubby species. Sedlitzia rosmarinus is resistant to salinity that plays an important role in soil conservation. In this research, RAPD molecular markers were used to evaluate the genetic diversity among 85 plant samples from 17 populations of S. rosmarinus. Five primers were used to perform RAPD reactions and a total of 400 DNA bands were obtained. After forming 0 and 1 matrix with the help of GenAlex 6.41 and UPGMA methods using SHAN clustering model, NTSYS Ver. 2.02 software was used to interpret RAPD analysis data. Molecular analysis of variance (AMOVA) was performed to estimate genetic diversity out of 85 individuals. Out of the total diversity, it was calculated that, the highest amount of genetic diversity i.e. 69% was related to intra-population and 31% to inter-population diversity, respectively.