Effect of Cod Liver Oil and Vitamin C Enriched Artemia on Growth, Survival and Stress Resistance of Yellowfin Seabream (Acanthopagrus latus) Larvae

A 36-day rearing trial was conducted to examine the enrichment of Artemia fransiscana nauplii with fatty acid and C (Ascorbyl-6-palmitate) on growth performance, survival and stress resistance of yellowfin seabream, Acanthopagrus latus larvae. Yellowfin seabream larvae at the first exogenous feeding with 53±0.01 mg body weight and 0.84±0.08 mm length were randomly distributed into 5 treatments and three replicate were assigned to each diet. The test treatments were as follows: larvae fed Cod liver oil + 5 %, 10 % and 15 % (w/w) vitamin C enriched Artemia nauplii (C1, C2 and C3 groups, respectively); larvae fed fatty acid without vitamin (HUFA group) and non-enriched Artemia (control). All treatments fed Rotifer for 5 days and then fed non-enriched Artemia for 10 days after first feeding and then fed enriched Artemia and commercial artificial feed for 21 days. At day 36, submersion in salt water (0 ppt for 2 hours) and cold water (15 ºC for 2 hours) was performed to evaluate larvae resistance to salinity and temperature stress. Growth parameters were analyzed at the end of the experiment. The highest growth was found in control group at the end of the experimental trial, but growth factors were not significantly different between groups (P<0.05). Survival was significantly different between control and other group from day 17 that fed enriched Artemia (P<0.05). Stress tolerance was not significantly different at 0 ppt and 15ºC. There was no comparable different in cortisol, total protein and glucose under stress conditions (P<0.05). These results indicated that the enrichment of Artemia with essential fatty acids and vitamins C can affect survival in Yellowfin seabream Acanthopagrus latus larvae.