The effect of aerobic training on expression of asprosin in pancreas of male rat diabetic

Asprosin is a counter-regulatory protein hormone of insulin and is associated with chronic diseases such as obesity and diabetes. Therefore, the aim of this study is to investigate the effect of aerobic exercise on the expression of the asprosin gene in the pancreatic tissue of male rats with diabetes.

32 male Wistar rats were randomly divided into 4 groups: control (n=8), exercise (n=8), diabetes (n=8), and diabetes+exercise (n=8) after inducing diabetes with STZ. After getting familiar with the training protocol, the rats in the training group performed the aerobic training protocol 5 times a week for eight weeks. After completing the protocol, pancreatic tissue was extracted and RT-PCR method was used to measure RNA and ELISA method was used to measure asprosin protein expression. Analysis of variance test was used to analyze the data (P<0.05).

The results showed that induction of diabetes significantly increases the expression level of asprosin gene and protein in pancreatic tissue (P<0.001). Eight weeks of aerobic training significantly reduced asprosin protein expression in the exercise group (P=0.019) but the decrease in gene and protein expression of asprosin in the diabetes+exercise group was not significant compared to the diabetes group.

Aerobic exercise reduced the level of asprosin in pancreatic tissue, which was increased due to diabetes, but was not able to return it to its original level. It seems that the effect of exercise is independent of the cellular and molecular pathways in the pancreatic tissue and plays a role mostly through the cell signaling pathways in the muscle, and in the damaged cells of the pancreatic tissue due to diabetes, in relation to asprosin addition to exercise, it is necessary to other treatment methods.