Prevalence of extended-spectrum beta-lactamases (ESBL) type’s blaVEB-1 and blaCTX-M-9 in Escherichia coli strains isolated from clinical samples by PCR technique

Escherichia coli (E. coli) is one of the bacteria that produce the enzyme β-Lactamase. This bacterium belongs to the Enterobacteriaceae family which causes urinary tract infections, nosocomial infections, and neonatal meningitis. This study aimed to evaluate the CTX-M9 and VEB 1 genes producers of β-lactamase in urine samples.

This study isolated 180 E. coli strains from urine samples at Miandoab hospitals. Antiprogram test was performed for antibiotic susceptibility and resistance. To identify ESBL-producing strains and blaCTX-M-9 and blaVEB-1, combined discs as well as PCR technique were applied.

The outcomes of the present study showed that 91 strains (51%) were positive for ESBL production. The resistance of E. coli was 56 and 47% to Nalidixic acid and CO-trimoxazole, respectively. The susceptibility to ciprofloxacin was 63%. blaVEB-1 gene with 51% was reported as the highest gene expressed in ESBL producing strains.

blaVEB-1 and blaCTX-M-9 were the most prevalent isolates in ESBL, respectively and these enzymes played an imperative role in resistance to beta-lactam antibiotics.