The Effect of High-intensity Interval Training on Chemrine and Adipsin Gene Expression of liver Tissue in Animal of Steatosis

Some adipokines are involved in the pathogenesis of nonalcoholic fatty liver disease. The aim of this study was to investigate the effect of high-intensity interval training on Chemrine and Adipsin gene expression of liver tissue in animal of steatosis. In this experimental study, 32 rats (weighing 200-250 gr) were selected and randomly divided into 4 groups including healthy control, fatty liver, HIIT and fatty liver + HIIT group. Rats were infected with fatty liver by oral tetracycline at a dose of 140 mg/kg (soluble in 2 ml of water) for 7 days. HIIT protocol contained 5 intervals 2 min running session at 85–90% of VO2max followed by 1 min running at 30–40% of VO2max between each session, five sessions per week for 5 weeks. The Chemrine and Adipsin gene expression in the liver tissue samples was measured by Real Time PCR. Data were analyzed by One-way ANOVA and Tukey post hoc tests at the P <0.05. The results showed that the Chemrine and Adipsin gene expression of liver tissue was significantly higher in fatty liver group than the healthy control group (P=0.001). Chemrine and Adipsin gene expression in liver tissue was significantly lower in HIIT and fatty liver + HIIT group’s than the fatty liver group (P=0.001). It seems that the HIIT training program can help improve the levels of adipokines in fatty liver disease, so this training method is recommended for its benefits in fatty liver disease.