Identification of bacterial agents involved in tumor formation on plum, sweet cherry and apple trees

During 2018 and 2019, a survey was carried out on the detection of bacterial crown gall disease on plum, sweet cherryDuring 2018 and 2019, a survey was carried out on detection of bacterial crown gall disease on plum, sweet cherry and apple trees in Urmia, Khoy, Salmas and Naghadeh in West Azarbaijan province. In total, 32 isolates causing gall on the crown of tomato seedlings were selected to identify further characteristics. Pathogenic strains were gram-negative, motile, and aerobic. In some of the phenotypic characteristics, differences were observed compared to the previous reports. These results indicate the existence of heterogeneity among Iranian strains. Molecular identification of strains was based on virD2, ipt, and virC genes using four specific primer pairs. The results of this research showed that the tested stone and pome fruit trees were infected with Agrobacterium tumefaciens No interference was observed in the function of the mentioned primers in the multiplex polymerase chain reaction. Thus, this accurate identification method is recommended by saving time and consumption of molecular materials.  .