Association between Pre-miR-3131 rs57408770 and Pri-miR-34b/c rs4938723 Gene Polymorphisms and the Risk of Non-Hodgkin Lymphoma

Lymphoma is the seventh most common malignancy worldwide, which is divided into types of neoplasms, of which three types, Hodgkin's lymphoma (HL), Non-Hodgkin's lymphoma (NHL), and Burkitt's lymphoma are the most common. miRNAs are regulators of gene expression that are involved in physiological processes and are involved in cancer. In the present study, the polymorphisms of pre-miR-3131 rs57408770 and Pri-miR-34b/c rs4938723 genes in patients with Non-Hodgkin's lymphoma were compared with healthy controls.

In this case-control study, 359 specimens were studied, of which 173 were (NHL) patients and 186 were healthy controls. Patients were selected from the patients referred to the cancer clinic of Ali Ibn Abi Talib Hospital. The DNA genome was extracted from the peripheral blood sample by the salting out method. The variants of Pri-mir-34b/c rs4938723 and pre-miR-3131 rs57408770 genotypes were analyzed by PCR-RFLP method.

The results showed that Pri-miR-34b/c rs4938723 homozygous polymorphism increased the risk of (NHL) in codominant and Recessive models. (OR= 2.70, 95% CI =1.13-4.43, P=0.025, OR=2.91, CI 95% =1.17-7.42, P=0.018 respectively). Pre-miR-3131 rs57408770 polymorphism increased the risk of (NHL) in homozygous states in codominant and recessive models. (OR=2.26, 95% CI=1.21-4.25, P=0.006, respectively).

The results showed a significant relationship between pre-miR-3131 rs57408770 polymorphism, Pri-miR-34b / c rs4938723, and (NHL) disease in the Iranian population.