Design and Fabrication of Modified DNA-Gp Nano-Biocomposite Electrode for Industrial Dye Measurement and Optical Confirmation

Indigo carmine dye is used in food and textile industries and has been proven to harm living organisms due to its binding to DNA in living organisms. In this research, a modified electrode by DNA/Gp nanocomposite on glassy carbon was designed and studied to measure this dye in actual samples. The results show the sensitivity of the modified electrode to this dye compared with the electrode without DNA/Gp nanocomposite. SEM, FT-IR, fluorescence, and electrochemical techniques were used to fabricate and examine nanocomposite and its reactions. The studies indicate that the electrochemical reaction is under adsorption control. The detection limit is 8.5×10-7 M, and the repeatability and stability of the electrode are at the optimal level.