Effect of explants and concentrations of plant growth regulators on callus induction In Ecotypes Of Ferula Assa-Foetida L

Some important herbs have been overly utilized in all over the world, which threatening them as endangered species. In this regard, in vitro culture has efficiently improved production and propagation rates of these plants. Accordingly, this research aimed to determine the best genotype, and explant for callus induction, as well as the effect of optimum concentration of hormones on traits including length, width, height and the callus volume of the explants derived from Ferula assa-foetida seedlings that have been grown in jars. Therefore, this experiment has been implemented as factorial in completely randomized design with at least 3 replications. Purposefully, the explants were placed in MS media supplemented with different levels of BAP and NAA. After 40 days, the traits including the length, width and volume of the callus were measured for induced callus. Given the mean comparison, the maximum size of target traits have been achieved for root explants cultured medium supplemented with 2 mg.L-1 BAP and 1 mg.L-1 NAA, while none of calluses derived from leaf and stem explants survived. Among the genotypes, the Kerman demonstrated higher callus growth rate than the Yazd, though this trait intensively increased through the time of culture. In addition, the calluses derived from Isfahan showed low growth rate, so it was removed from the present study.