Histopathological effect of mixed probiotic(Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus heloticus) on copper sulfate induced lesions in the lung tissue of male rats

Heavy metals can have adverse effects on humans due to their toxic nature. One of the most important strategies to adjust the microbiota balance and improve the health status is the use of probiotics as beneficial live microorganisms.The purpose of this research is to investigate the effect of probiotic mixture of Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus heloticus on the induced effects of copper sulfate in the lung tissue of male rats.methods and Materials:In this experimental study, 21 male Wistar rats were divided into 3 groups of 7: control, receiving copper sulfate and copper sulfate + probiotic mixture. Copper sulfate (200 mg/kg) three days in a row and probiotics (109 CFU/Ml) were gavage to the rats for 35 days. The samples were photographed and studied by hematoxylin-eosin staining and light microscopy.Examining the morphological changes of the lung tissue in the probiotic treatment group showed a reduction in tissue damage compared to the copper sulfate group. According to the findings of this research, probiotics can probably be used as a new strategy in a wide range of medicinal products. The goals of prevention or improvement of treatment methods were used.