A comparative study on the scolicidal effect of amino-bromophenylcoumarin and amino-hydroxyphenylcoumarin on hydatid cyst protoscolexes in vitro

Echinococcus granulosus is the causative agent of hydatid cysts, which are distributed worldwide. Nowadays, much research is being done to inactivate hydatid cyst protoscoleces. This study was conducted with the aim to compare the scolicidal effect of two chemical compounds 12 and 2 on hydatid cyst protoscoleces in vitro.

The scolicidal activity of two amini-hydrox-phenylcoumarin and amini-bromo-phenylcoumarin at concentrations of 1.2, 1.5, 1.10 and 1.100 after 15, 30 and 60 minutes of incubation was tested separately and each of the experiments was repeated three times. The viability of protoscoleces was checked with 0.1% eosin dye under a light microscope. Subsequently data were analyzed using GraphPad version 5 software and two-way repeated measures ANOVA and values  (P > 0.05) was considered a significant level.

The results of this study showed that the chemical compound amini-bromophenylcoumarin at a concentration of 1.10 destroyed 100% of protoscoleces after 60 minutes of exposure compared to the control group, but amini-hydroxophenyl-coumarin, at a concentration of 1.100 could destroy 98% of protoscolecia after 60 minutes of exposure.

The results of the present study showed that amini-bromophenylcoumarin had stronger protoscolicidal effects than amini-hydroxophenyl-coumarin. However, further studies and in vivo studies are needed to evaluate the efficacy of these chemical compounds.