Bioinformatics analysis and investigation of PI3K/AKT gene expression in exposure to the active substance of cinnamon in cinnamomum plant

Cinnamomum is a type of tonic spice that has had a special place in traditional medicine for a long time due to its effects on body health. Even today, with the discovery of its wide therapeutic properties, it is used in many countries. The main components of Cinnamomum, including cinnamaldehyde, can play a role in the treatment of breast cancer. In this study, we investigated the antioxidant effect and anticancer properties of the active ingredient in cinnamon in breast cancer. The dose of the active ingredient of cinnamon was determined and checked by the MTT test method. The effects of cinnamon active substance on PI3K/AKT signaling pathway were investigated using molecular docking and real-time PCR methods. The results were that the dose of the effective substance was equal to 0.093 mg/ml and among the four proteins, AKT gene was selected due to the highest and best reaction at the protein level and the expression of AKT gene was very low in the presence of cinnamon. According to the effects, activity and antioxidant properties of the active substance of cinnamon, taking into account their side effects in vitro and in silico, this active substance of the plant can be used as an inhibitor of the PI3K/AKT signaling pathway and also it can be used as a Malignant tumor preventer.